PCR Detection of Microbial Pathogens

PCR Detection of Microbial Pathogens : Methods and Protocols

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Description

Hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis.show more

Product details

  • Hardback | 352 pages
  • 157.5 x 236.2 x 27.9mm | 657.72g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 1, black & white illustrations
  • 1588290492
  • 9781588290496

Review quote

"...PCR Detection of Microbial Pathogens is useful, comprehensive and practical guide for everyone who wants to use PCR for detection of the above mentioned pathogens and, at the same time, an inspiration for development of new PCR detection systems." -Folia Microbiologicashow more

Back cover copy

Because the number of published PCR procedures is already large and rising steadily, there is a need to critically evaluate and eventually standardize its methodology so that laboratory diagnosticians can select optimal techniques for their own work. In PCR Detection of Microbial Pathogens, hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. Described in step-by-step detail to ensure successful experimental results, these proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis. Each article details a validated technique that explains the principles involved, provides stepwise instructions and a list of materials, and includes notes describing problems that may arise, as well as possible alternative procedures. Authoritative and easy-to-use, PCR Detection of Microbial Pathogens offers microbiologists, veterinary diagnosticians, and food analysts powerful state-of-the-art tools designed not only to expand their knowledge of the potential of DNA-based diagnostic methods, but also to help select those techniques best suited to their specific practical needs.show more

Table of contents

Part I. Reviews Specificity and Performance of Diagnostic PCR Assays Konrad Sachse Pre-PCR Processing of Samples Peter Radstrom, Rickard Knutsson, Petra Wolffs, Maria Dahlenborg, and Charlotta Lofstrom Critical Aspects of Standardization of PCR Jeffrey Hoorfar and Nigel Cook PCR Technology and Applications to Zoonotic Food-Borne Bacterial Pathogens Peter Stephensen Lubeck and Jeffrey Hoorfar Part II. Protocols Detection, Identification, and Subtyping of Actinobacillus pleuropneumoniae Joachim Frey Identification and Differentiation of Brucella abortus Field and Vaccine Strains by BaSS-PCR Darla R. Ewalt and Betsy J. Bricker Isolation of Campylobacter and Identification by PCR Mark D. Englen, Scott R. Ladely, and Paula J. Fedorka-Cray Detection and Differentiation of Chlamydiae by Nested PCR Konrad Sachse and Helmut Hotzel Detection of Toxigenic Clostridia Michel R. Popoff PCR-Based Detection of Coxiella burnetii from Clinical Samples Mustapha Berri, Nathalie Arricau-Bouvery, and Annie Rodolakis Detection and Subtyping of Shiga Toxin-Producing Escherichia coli (STEC) Peter Gallien Detection of Listeria monocytogenes Using a PCR/DNA Probe Assay Louise O'Connor Detection of Leptospira interrogans John W. Lester and Rance B. LeFebvre Detection of Pathogenic Mycobacteria of Veterinary Importance Robin A. Skuce, M. Siobhan Hughes, Malcolm J. Taylor, and Sydney D. Neill Multiplex PCR of Avian Pathogenic Mycoplasmas Mazhar I. Khan Detection and Differentiation of Ruminant Mycoplasmas Helmut Hotzel, Joachim Frey, John Bashiruddin, and Konrad Sachse Detection of Mycoplasma hyopneumoniae from Clinical Samples and Air Marylene Kobisch and Joachim Frey PCR-Detection of Hemophilus paragallinarum, Hemophilus somnus, Mannheimia (Pasteurella) hemolytica, Mannheimia spp., Pasteurella trehalosi, and Pasteurella multocida Henrik Christensen, Magne Bisgaard, Jesper Larsen, and John Elmerdahl Olsen Detection of Salmonella spp. Burkhard Malorny and Reiner Helmuth Detection of Toxoplasma gondii Jonathan M. Wastling and Jens G. Mattsson PCR-Derived Methods for the Identification of Trichinella Parasites from Animal and Human Samples Edoardo Pozio and Giuseppe La Rosa Detection of Pathogenic Yersinia enterocolitica by a Swab Enrichment PCR Procedure Rickard Knutsson and Peter Radstrom Indexshow more