Opioid Research
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Opioid Research : Methods and Protocols

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Description

Opioid research is one of the multidisciplinary research areas that involve advanced techniques ranging from molecular genetics to neuropharmacology, and from behavioral neuroscience to clinical medicine. In current opioid research, it has become increasingly important to use multiple approaches at molecular, cellular, and system levels for investigations on a specific opio- related target system. That often requires understanding and applying cro- field techniques and methods for the success of one's research projects. Through its broad spectrum of coverage, Opioid Research: Methods and Protocols provides a comprehensive collection of major laboratory methods and protocols in current opioid research, covering topics from molecular and genetic techniques to behavioral analyses of animal models, and then to clinical practice. It will serve as a convenient reference book from which those involved in opioid research will learn or perfect the necessary cross-field techniques. The detailed methods and protocols described in Opioid Research: Methods and Protocols have each been successfully applied in current opioid research. Part I provides molecular techniques for the cloning and expression of opioid receptors, and for the quantitative characterization of their signaling pathways. Part II includes primary techniques for mapping the distributions and detecting the expression levels of opioid receptors, opioid peptides, and their messages in brain tissues and in individual cells. Part III deals with methods for creating in vitro receptor models and in vivo animal models to study opioid functions. Part IV describes practical applications of opioids in clinical medicine for the treatment of pain and opioid addiction.
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Product details

  • Paperback | 307 pages
  • 154.94 x 230.12 x 18.54mm | 471.73g
  • Totowa, NJ, United States
  • English
  • Softcover reprint of hardcover 1st ed. 2003
  • X, 307 p.
  • 161737329X
  • 9781617373299

Back cover copy

Opioid research has grown enormously over the last decade, and with it, the need to apply multiple techniques from a variety of disciplines. In Opioid Research: Methods and Protocols, expert researchers and physicians provide a comprehensive collection of the major cross-disciplinary methods and protocols used in current opioid research, covering topics from molecular and genetic techniques, to behavioral analyses of animal models, to clinical practices. The well-practiced authors describe their best molecular techniques for the cloning and expression of opioid receptors, and for the quantitative characterization of their signaling pathways, as well as for mapping the distribution and detecting the expression levels of opioid receptors, opioid peptides, and their messages, in both brain tissues and individual cells. Also included are methods for the creation of in vitro and in vivo animal models to study opioid functions, as well as the clinical applications in the treatment of pain and opioid addiction. Each proven technique contains step-by-step instructions, explanations of the basic science involved, and notes on avoiding pitfalls.
Comprehensive and authoritative, Opioid Research: Methods and Protocols offers researchers an indispensable guide to all the major multidisciplinary techniques needed for successful research in opioid biology and medicine today.
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Table of contents

Part I. Molecular Characterization of Opioid Receptors and Signaling Pathways

Molecular Cloning of Opioid Receptors by cDNA Library Screening
Ying-Xian Pan

Expression of Opioid Receptors in Mammalian Cell Lines
Ying-Xian Pan

Assessing Opioid Regulation of Adenylyl Cyclase Activity in Intact Cells
Deepak R. Thakker, Hatice Z. Ozsoy, and Kelly M. Standifer

Analysis of Opioid-Induced Kinase Activation, Lan Ma

Study of Opioid Receptor Phosphorylation Using Cell-Labeling Method with 32P-Orthrophosphate
Jia Bei Wang

Opioid Receptor Coupling to GIRK Channels: In Vitro Studies Using a Xenopus Oocyte Expression System and In Vivo Studies on Weaver Mutant Mice
Kazutaka Ikeda, Mitsunobu Yoshii, Ichiro Sora, and Toru Kobayashi

Identification of Alternatively Spliced Variants from Opioid Receptor Genes
Ying-Xian Pan

Part II. Mapping and Detection of Endogenous Opioids

Immunohistochemical Localization of m-, d- and k-Opioid Receptors Within the Antinociceptive Brainstem Circuits
Alexander E. Kalyuzhny

In Situ Hybridization in Neural Tissues
Howard B. Gutstein

Quantitative Single-Cell RT-PCR for Opioid Receptors and Housekeeping Genes
Seth C. Silbert

Gene Arrays and Proteomics: A Primer
Lionel Mouledous and Howard B. Gutstein

Part III. Model Systems for Studies on Opioid Functions

Opioid Receptor Oligomerization: Detection and Functional Characterization of Interacting Receptors
Ivone Gomes, Julija Filipovska, and Lakshmi A. Devi

Recombinant Opioid Receptors: Structure-Function Relationship
Julija Filipovska, Ivone Gomes, Wei Xu, Chongguang Chen, Lee-Yuan Liu-Chen, and Lakshmi A. Devi

Receptor Knock-Out and Gene Targeting: Generation of Knock-Out Mice
Ichiro Sora, Kazutaka Ikeda, and Yuji Mishina

Pharmacological Interventions at theSpinal Cord: Intrathecal Injections
Daphne A. Robinson and Min Zhuo

Opioid Tolerance in Adult and Neonatal Rats
Zhizhong Z. Pan

Animal Models of Neuropathic Pain
William J. Martin, Laike St. A. Stewart, and Jason W. Tarpley

Place Conditioning to Study Drug Reward and Aversion
William A. Carlezon, Jr.

Opiate Self-Administration
Zheng-Xiong Xi and Elliot A. Stein

Part IV. Clinical Applications of Opioids

Acute, Chronic, and Cancer Pain: Clinical Management
Allen W. Burton

Clinical Treatment of Opioid Addiction and Dependence
Walter Ling, Richard A. Rawson and Margaret Compton

Index
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