Nucleosomes: Volume 170
- Hardback | 683 pages
- 154 x 230 x 32mm | 1,079.56g
- 18 Sep 1989
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
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Table of contents
R.D. Kornberg, Preparation of Chromatin and Nucleosomes: General Considerations.
P. Oudet, E. Weiss, and E. Regnier, Preparation of Simian Virus 40 Minichromsomes.
A. Dean, D.S. Pederson, and R.T. Simpson, Isolation of Yeast Plasmid Chromatin.
T.M. Herman, Affinity Isolation of Replicating Simian Virus 40 Replicating Minichromosomes.
Chromatin and Nucleosomes: Analytical Techniques:
H. Noll and M. Noll, Sucrose Gradient Techniques and Applications to Nucleosome Structure.
S.Y. Huang and W.T. Garrard, Electrophoretic Analyses of Nucleosomes and Other Protein-DNA Complexes.
J.M. Sogo and F. Thoma, Electron Microscopy of Chromatin.
P. Oudet, P. Schultz, J.-C. Homo, and P. Colin, Electron Microscopy of Simian Virus 40 Minichromosomes.
C.L.F. Woodcock, Immunoelectron Microscopy of Nucleosomes.
J.-R. Daban and C.R. Cantor, Use of Fluorescent Probes to Study Nucleosomes.
M. Bustin, Preparation and Application of Immunological Probes for Nucleosomes.
S. Muller and M.H.V. Van Regenmortel, Use of Antihistone Antibodies with Nucleosomes.
L.C. Lutter, Digestion of Nucleosomes with Deoxyribonucleases I and II.
C. Wu, Analysis of Hypersensitive Sites in Chromatin.
M.E. Cusick, P.M. Wassarman, and M.L. DePamphilis, Application of Nucleases to Visualizing Chromatin Organization at Replication Forks.
M. Bellard, D. Dretzen, A. Giangrande, and P. Ramain, Nuclease Digestion of Transcriptionally Active Chromatin.
J.M. Gottesfeld, Analysis of RNA Polymerase III Transcription in Vitro Using Chromatin and Cloned Gene Templates.
I.L. Cartwright and S.C.R. Elgin, Nonenzymatic Cleavage of Chromatin.
J.O. Thomas, Chemical Radiolabeling of Lysines that Interact Strongly with DNA in Chromatin.
A.D. Mirzabekov, S.G. Bavykin, A.V. Belyavsky, V.L. Karpov, O.V. Preobrazhenskaya, V.V. Shich, and K.K. Ebralidse, Mapping DNA-Protein Interaction by Cross-Linking.
S.A. Nedospasov, A.N. Shakhov, and G.P. Georgiev, Analysis of Nucleosome Positioning by Indirect End-Labeling and Molecular Cloning.
D. Rhodes, R.S. Brown, and A. Klug, Crystallization of Nucleosome Core Particles.
Histones: Preparation and Analysis:
C. von Holt, W.F. Brandt, H.J. Greyling, G.G. Lindsey, J.T. Retief, J. de A. Rodrigues, S. Schwager, and B.T. Sewell, Isolation and Characterization of Histones.
R.D. Cole, Purification and Analysis of H1 Histones.
R.W. Lennox and L.H. Cohen, Analysis of Histone Subtypes and Their Modified Forms by Polyacrylamide Gel Electrophoresis.
J.O. Thomas, Chemical Cross-Linking of Histones.
Assembly and Reconstitution of Chromatin:
D. Rhodes and R.A. Laskey, Assembly of Nucleosomes and Chromatin in Vitro.
A. Stein, Reconstitution of Chromatin from Purified Components.
A. Shimamura, B. Jessee, and A. Worcel, Assembly of Chromatin with Oocyte Extracts.
L. Sealy, R.R. Burgess, M. Cotten, and R. Chalkley, Purification of Xenopus Egg Nucleoplasmin and Its Use in Chromatin Assembly in Vitro.
B. Neubauer and W. H~adorz, Analysis of Nucleosome Positioning by in Vitro Reconstitution.
"Its four sections cover a broad range of procedures designed to take you from cell to gel... The longest section, that on analytical techniques, starts with a review of the theory of sedimentation. Sucrose gradients are featured. Most of the other chapters here include preparatory methods, often for a specific organism or tissue."
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