Nuclear Transfer Protocols : Cell Reprogramming and Transgenesis

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.
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Part I. Nuclear Transfer

Origin and Progress of Nuclear Transfer In Nonmammalian Animals
Marie A. Di Berardino

A. Culture and Maintenance of Nuclear Transfer Embryos

In Vitro Maturation and Embryo Production in Cattle
Marc-Andre Sirard and Karine Coenen

Activation of Oocytes After Nuclear Transfer
Zoltan Machaty

Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos
Bjoern Heindryckx, Josiane Van der Elst, and Marc Dhont

Maintenance of Pregnancy in Pigs With Limited Viable Embryos
Tim King and Paul A. De Sousa

Health and Neonatal Care of Bovine Clones
Jacqui T. Forsyth and David N. Wells

B. Nuclear Transfer Methods

Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice
Young Gie Chung, Shaorong Gao, and Keith E. Latham

Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors
Kazuchika Miyoshi, Sezen Arat, and Steven L. Stice

A Double Nuclear Transfer Technique for Cloning Pigs
Irina A. Polejaeva, Shawn Walker, and Keith Campbell

Nuclear Transfer in Nonhuman Primates
Shoukhrat M. Mitalipov and Don P. Wolf

Cloning of Exotic/Endangered Species: Desert Bighorn Sheep
James 'Buck' Williams, Taeyoung Shin, Ling Liu, Gabriela Flores-Foxworth, Juan Romano, Alice Blue-McClendon, Duane Kraemer, and Mark E. Westhusin

Handmade Somatic Cell Cloning in Cattle
Gabor Vatja, Ian M. Lewis, and R. Tayfur Tecirlioglu

C. Transgenesis

Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells
A. John Clark, Sarah Burl, and Chris Denning

Transgenesis Using Nuclear Transfer in Goats
Anthoula Lazaris, Rebecca Keyston, Costas N. Karatzas, and Carol L. Keefer

Part II. Reprogramming Nuclei

Nuclear Reprogramming: AnOverview
Takashi Tada

Expression of Imprinted Genes in Cloned Mice
Takashi Kohda, Fumitoshi Ishino, and Atsuo Ogura

Nuclear Remodeling Assay in Xenopus Egg Extract
Koichi Gonda and Nobuaki Kikyo

In Vitro Reprogramming of Nuclei and Cells
Anne-Mari Hakelien, Thomas Kuntziger, Kristine G. Gaustad, Anne Marstad, and Philippe Collas

Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells
Daniele Pralong and Paul J. Verma

Gene Expression and Methylation Patterns in Cloned Embryos
Christine Wrenzycki, Doris Herrmann, Claudia Gebert, Joseph W. Carnwath, and Heiner Niemann

Part III. Concluding Remarks

Are There Any Normal Clones?
Ian Wilmut

Future and Applications of Cloning
Alan O. Trounson

Index
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"...an essential comprehensive handbook for both research and commercials labs involved in nuclear transfer, and will be of interest to molecular and stem cell biologists and biotechnologists involved with reprogramming, nuclear transfer and transgensis." -Biotech-online
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