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Nuclear Transfer Protocols : Cell Reprogramming and Transgenesis
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Description
Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.
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Product details
- Hardback | 338 pages
- 157.5 x 233.7 x 22.9mm | 521.64g
- 04 Aug 2006
- Humana Press Inc.
- Totowa, NJ, United States
- English
- 2006 ed.
- XII, 338 p.
- 1588292800
- 9781588292803
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Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.
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Table of contents
Part I. Nuclear Transfer
Origin and Progress of Nuclear Transfer In Nonmammalian Animals
Marie A. Di Berardino
A. Culture and Maintenance of Nuclear Transfer Embryos
In Vitro Maturation and Embryo Production in Cattle
Marc-Andre Sirard and Karine Coenen
Activation of Oocytes After Nuclear Transfer
Zoltan Machaty
Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos
Bjoern Heindryckx, Josiane Van der Elst, and Marc Dhont
Maintenance of Pregnancy in Pigs With Limited Viable Embryos
Tim King and Paul A. De Sousa
Health and Neonatal Care of Bovine Clones
Jacqui T. Forsyth and David N. Wells
B. Nuclear Transfer Methods
Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice
Young Gie Chung, Shaorong Gao, and Keith E. Latham
Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors
Kazuchika Miyoshi, Sezen Arat, and Steven L. Stice
A Double Nuclear Transfer Technique for Cloning Pigs
Irina A. Polejaeva, Shawn Walker, and Keith Campbell
Nuclear Transfer in Nonhuman Primates
Shoukhrat M. Mitalipov and Don P. Wolf
Cloning of Exotic/Endangered Species: Desert Bighorn Sheep
James 'Buck' Williams, Taeyoung Shin, Ling Liu, Gabriela Flores-Foxworth, Juan Romano, Alice Blue-McClendon, Duane Kraemer, and Mark E. Westhusin
Handmade Somatic Cell Cloning in Cattle
Gabor Vatja, Ian M. Lewis, and R. Tayfur Tecirlioglu
C. Transgenesis
Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells
A. John Clark, Sarah Burl, and Chris Denning
Transgenesis Using Nuclear Transfer in Goats
Anthoula Lazaris, Rebecca Keyston, Costas N. Karatzas, and Carol L. Keefer
Part II. Reprogramming Nuclei
Nuclear Reprogramming: AnOverview
Takashi Tada
Expression of Imprinted Genes in Cloned Mice
Takashi Kohda, Fumitoshi Ishino, and Atsuo Ogura
Nuclear Remodeling Assay in Xenopus Egg Extract
Koichi Gonda and Nobuaki Kikyo
In Vitro Reprogramming of Nuclei and Cells
Anne-Mari Hakelien, Thomas Kuntziger, Kristine G. Gaustad, Anne Marstad, and Philippe Collas
Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells
Daniele Pralong and Paul J. Verma
Gene Expression and Methylation Patterns in Cloned Embryos
Christine Wrenzycki, Doris Herrmann, Claudia Gebert, Joseph W. Carnwath, and Heiner Niemann
Part III. Concluding Remarks
Are There Any Normal Clones?
Ian Wilmut
Future and Applications of Cloning
Alan O. Trounson
Index
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Origin and Progress of Nuclear Transfer In Nonmammalian Animals
Marie A. Di Berardino
A. Culture and Maintenance of Nuclear Transfer Embryos
In Vitro Maturation and Embryo Production in Cattle
Marc-Andre Sirard and Karine Coenen
Activation of Oocytes After Nuclear Transfer
Zoltan Machaty
Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos
Bjoern Heindryckx, Josiane Van der Elst, and Marc Dhont
Maintenance of Pregnancy in Pigs With Limited Viable Embryos
Tim King and Paul A. De Sousa
Health and Neonatal Care of Bovine Clones
Jacqui T. Forsyth and David N. Wells
B. Nuclear Transfer Methods
Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice
Young Gie Chung, Shaorong Gao, and Keith E. Latham
Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors
Kazuchika Miyoshi, Sezen Arat, and Steven L. Stice
A Double Nuclear Transfer Technique for Cloning Pigs
Irina A. Polejaeva, Shawn Walker, and Keith Campbell
Nuclear Transfer in Nonhuman Primates
Shoukhrat M. Mitalipov and Don P. Wolf
Cloning of Exotic/Endangered Species: Desert Bighorn Sheep
James 'Buck' Williams, Taeyoung Shin, Ling Liu, Gabriela Flores-Foxworth, Juan Romano, Alice Blue-McClendon, Duane Kraemer, and Mark E. Westhusin
Handmade Somatic Cell Cloning in Cattle
Gabor Vatja, Ian M. Lewis, and R. Tayfur Tecirlioglu
C. Transgenesis
Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells
A. John Clark, Sarah Burl, and Chris Denning
Transgenesis Using Nuclear Transfer in Goats
Anthoula Lazaris, Rebecca Keyston, Costas N. Karatzas, and Carol L. Keefer
Part II. Reprogramming Nuclei
Nuclear Reprogramming: AnOverview
Takashi Tada
Expression of Imprinted Genes in Cloned Mice
Takashi Kohda, Fumitoshi Ishino, and Atsuo Ogura
Nuclear Remodeling Assay in Xenopus Egg Extract
Koichi Gonda and Nobuaki Kikyo
In Vitro Reprogramming of Nuclei and Cells
Anne-Mari Hakelien, Thomas Kuntziger, Kristine G. Gaustad, Anne Marstad, and Philippe Collas
Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells
Daniele Pralong and Paul J. Verma
Gene Expression and Methylation Patterns in Cloned Embryos
Christine Wrenzycki, Doris Herrmann, Claudia Gebert, Joseph W. Carnwath, and Heiner Niemann
Part III. Concluding Remarks
Are There Any Normal Clones?
Ian Wilmut
Future and Applications of Cloning
Alan O. Trounson
Index
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Review quote
"...an essential comprehensive handbook for both research and commercials labs involved in nuclear transfer, and will be of interest to molecular and stem cell biologists and biotechnologists involved with reprogramming, nuclear transfer and transgensis." -Biotech-online
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