Nonisotopic DNA Probe Techniques
Many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as phosphorous and iodine. This book aims to bring these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited for their application and to maximize its advantage by following the instructions provided. The book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluroescent and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapter presents background information and detailed, easy-to-follow experimental procedures for labelling and detection.
- Paperback | 358 pages
- 152.4 x 228.6 x 22.35mm | 630.49g
- 30 Apr 1992
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
- references, index
Table of contents
Nucleic acid hybridization test formats, strategies and applications, C. Kessler; nonradioactive labeling methods for nucleic acids, detection methods, E. Templeton, et al; alkaline phosphatase/time-resolved fluorescence, R.E. Geiger; alkaline phosphatase/bioluminescence, A. Tumulo, et al; alkaline phosphatase/chemiluminescence, A. Rashtchian; alkaline phosphatase/colorimetry, I. Durant; horseradish peroxidase/enhanced chemiluminescence, P.C.Verlander; horseradish peroxidase/colorimetry, P. Balaguer, et al; glucose 6-phosphate dehydrogenase/bioluminescence, T. Lovgren, et al; lanthanide chelates/time-resolved fluorescence, E.P. Diamandis and T.K. Christopoulos; lanthanide chelates and multiple labelling strategies/time-resolved fluorescence, N.C. Nelson, et al; acridinium esters/ chemiluminescence, L.E. Morrison; energy transfer and fluorescence quenching methods.