Nitric Oxide Synthase: Characterization and Functional Analysis: Volume 31

Nitric Oxide Synthase: Characterization and Functional Analysis: Volume 31

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Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field.
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Product details

  • Hardback | 354 pages
  • 192 x 244 x 24mm | 799.99g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0121853012
  • 9780121853013

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Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field.
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Table of contents

Enzyme Activity Measurements, Nitric Oxide Detection, and Tissue Localization:
Electron Paramagnetic Resonance Detection of Nitric Oxide in Biological Samples, L.G. Rochelle, H. Kruszyna, and R.P. Smith.
Electrochemical and Spectroscopic Methods of Nitric Oxide Detection, T. Malinski, E. Kubaszewski, and F. Kiechle.
Detection of S-Nitrosothiols, G.N. Welch, G.R. Upchurch, Jr., and J. Loscalzo.
Nitrosation of Proteins, Y.-Y. Zhang and J. Loscalzo.
Immunohistochemical Methods for Nitric Oxide Synthase, P.K. Nakane
NADPH Diaphorase Staining, T.M. Dawson and V.L. Dawson.
Immunohistochemical Visualization of Cyclic Nucleotides, J. de Vente, H.M. Young, and H.W.M. Steinbusch.
Detection of Downstream Components of Cyclic GMP Cascade by in Situ Hybridization Analysis, M.F. Goy, Z. Li, E.M. Zimmerman, and D.R. Repaske.
Detection of Heme Oxygenase-1 and -2 Transcripts by Northern Blot and in Situ Hybridization Analysis, J.F. Ewing.
Enzyme Expression, Purification, and Gene Regulation:
Purification of Endothelial Nitric Oxide Synthase, J.S. Pollock and M. Nakanev
Purification of Brain Nitric Oxide Synthase from Baculovirus Overexpression System and Determination of Cofactors, B. Mayer, P. Klatt, C. Harteneck, B.M. List, E.R. Werner, and K. Schmidt.
Prokaryotic Expression, Purification, and Characterization of Intact and Modular Constructs of Neuronal Nitric Oxide Synthase, B.S.S. Masters, K. McMillan, L.J. Roman, P. Martasek, and J. Nishimura.
Generation of Monoclonal Antibodies to Endothelial Nitric Oxide Synthase, J.S. Pollock and M. Nakane.
Genetic Modulation of Inducible Nitric Oxide Synthase Expression, E. Tzeng and T.R. Billiar.
Expression of Constitutive Brain Nitric Oxide Synthase and Inducible Nitric Oxide Synthase by Cultured Neurons and Glia, D. Minc-Golomb and J.P. Schwartz.
Structural Characterization of Human Neuronal Nitric Oxide Synthase Gene: Methodological Approach to a Complex Transcription Unit, A.H. Cheung and P.A. Marsden.
Transcriptional Control of Endothelial Nitric Oxide Synthase Gene Expression, W.C. Sessa.
Site-Directed Mutagenesis to Probe Endothelial Nitric Oxide Synthase Posstranslational Modifications, J. Liu and W.C. Sessa.
Assessment of Nitric Oxide-Mediated Functions at Cell and Organ Levels:
Nitric Oxide and Endothelial Regulation of Vascular Tone, F. Cosentino and T.F. Luscher.
Function of Nitric Oxide in Neuronal Cell Death, V.L. Dawson and T.M. Dawson.
Nitric-Oxide-Cyclic Guanoseine Monophosphate Pathway in Central Nervous system: In Vitro and in Vivo Investigations, M. Salter, E. Southam, and J. Garthwaite.
Detection and Contriution of Nitric Oxide to Endothelium Regulation of Microvascular Tone, U. Pohl.
Regulation of Cerebral Circulation by Nitric Oxide, F.M. Faraci
Nitric Oxide in NAD/NADH-Dependent Protein Modification, B. Brune and E.G. Lapetina.
Assessment of Synaptic Effects of Nitric Oxide in Hippocampal Neurons, C.F. Zorumski, S. Mennerick, and Y. Izumi.
Synaptic Transmission in Hippocampal Slice, E.M. Schuman
Redox Congeners of Nitric Oxide, N-Methyl-D-Aspartate Receptors, and Intracellular Calcium Ion, W.-K. Kim, J.S. Stamler, and S.A. Lipton
Cytosolic and Nuclear Calcium Imaging by Confocal Microscopy, M. Burnier, G. Centeno, and H.R. Brunner.
Effects of Nitrogen Monoxide on Cellular Iron Metabolism, D.R. Richardson and P. Ponka.
Index.
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Review quote

Praise for the Series: "Well-written, clear and detailed.... The authors do indeed offer the 'fine detail, tricks and short cuts' not usually found in the written word." --Trends in the Neurosciences
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About Mahin D. Maines

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public's awareness of diabetes. Conn's students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.
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