Nitric Oxide Protocols

Nitric Oxide Protocols

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It has been more than five years since the appearance of the first edition of Nitric Oxide Protocols. According to Medline, more than 32,500 publications dealing with nitric oxide have appeared in the primary literature in the int- vening time from 1999 to April 2004. These numbers attest to the continuing vitality of a field dealing with a deceptively simple, although extremely int- esting and promiscuous molecule, nitric oxide. Despite its apparent chemical simplicity, nitric oxide continues to amaze, with an extremely wide spectrum of functions and effects. In particular, the last few years have witnessed an explosion of nonclassical effects, involving the direct interaction of nitric - ide and related oxidized products with proteins to yield such novel combi- tions as nitrosylated and nitrated proteins. Another emergent area has been that of NO's clinical applications, including interesting developments related to the virus-mediated expression of nitric oxide synthase and the use of nitric oxide in gene therapy. In Nitric Oxide Protocols, I have gathered chapters dealing with the some of the latest methodologies for the measurement of nitric oxide.
Many of these deal with quantitation in biological fluids or tissues, and they cover a wide spectrum of technologies, including capillary electrophoresis, microcoaxial electrodes, the in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography-mass spectroscopy, in situ hybridization, and real-time polymerase chain reaction.
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Product details

  • Hardback | 248 pages
  • 154.9 x 233.7 x 22.9mm | 567g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2004
  • XII, 248 p.
  • 1588292371
  • 9781588292377

Review quote

From reviews of the first edition...

" excellent book...offers newcomers and experienced scientists a wealth of important practical information that will be of inestimable value both to newcomers and experienced research essential resource for every laboratory involved in NO-related work..." -Angewandte Chemie

"...a very useful handbook and definitely a must for researchers working at the bench in any field related one way or another to NO." -Psychoneuroendocrinology

"...This book is a must for laboratories with an interest in nitric oxide..." -British Journal of Anesthesia

"...will certainly find its way into many laboratories entering the exciting field of nitric oxide research. " -Quarterly Review of Biology
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Back cover copy

Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular Biology(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects.
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Table of contents

Measurement of Nitric Oxide by Reconversion of Nitrate/Nitrite to NO
Reinhard Berkels, Svenja Purol-Schnabel, and Renate Roesen

High-Resolution Capillary Electrophoresis of Nitrite and Nitrate in Biological Samples
Dmitri Y. Boudko

Nitrite and Nitrate Measurements in Human Urine by Capillary Electrophoresis
Edward Morcos and N. Peter Wiklund

In Vivo Nitric Oxide Measurements Using a Microcoaxial Electrode
Yoshiichiro Kitamura, Hirosuke Kobayashi, Kazuo Tanishita, and Kotaro Oka

Measurement of Exhaled Nitric Oxide
Peter A. Steerenberg and Jan G. C. van Amsterdam

Nitric Oxide Imaging in Neurons Using Confocal Microscopy
Xiaoxiang Zheng, Gangmin Ning, Dihui Hong, and Mu Zhang

Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Spectrometry
Dimitrios Tsikas

A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts
Hazel Sutherland, Olga Zolle, Roba Khundkar, Alec W. M. Simpson, Jonathan C. Jarvis, and Stanley Salmons

Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization
Olivier Braissant

Real-Time Polymerase Chain Reaction to Quantify mRNA for Endothelial Nitric Oxide Synthase
Yi Chu and Frank M. Faraci

Dioxygen-Dependent Metabolism of Nitric Oxide
Paul R. Gardner, Anne M. Gardner, and Craig K. Hallstrom

Proteomic Method for Identification of Tyrosine-Nitrated Proteins
Kulwant S. Aulak, Thomas Koeck, John W. Crabb, and Dennis J. Stuehr

Immunohistochemical Detection of S-Nitrosylated Proteins
Andrew J. Gow, Christiana W. Davis, David Munson, and Harry Ischiropoulos

Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Corpora Cavernosa for Erectile Dysfunction
Trinity J. Bivalacqua, Weiwen Deng, Hunter C. Champion, Wayne J. G. Hellstrom, and Philip J. Kadowitz

Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer Into the Nucleus Tractus Solitarius in Conscious Rats
Yoshitaka Hirooka and Koji Sakai

Gene Therapy in Cancer Via Use of a Retrovector Having a Tumor Specificity and Expressing Inducible Nitric Oxide Synthase
Masahide Kuroki

Gene Transfer of Nitric Oxide Synthase Via the Use of Adeno-Associated Virus Vectors
Yoshikazu Maeda, Kazuyuki Shimada, and Uichi Ikeda

Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer
Kathleen G. Raman, Richard A. Shapiro, Edith Tzeng, and Melina R. Kibbe

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