Next Generation Sequencing

Next Generation Sequencing : A Primer

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Description

Next Generation Sequencing demystifies the process of sequencing with a hands-on approach. Offering a theoretical and practical discussion of the how and why behind the kit culture, this publication instructs readers on the science behind the technology and the analysis and application of data. The primer includes a needed pros-and-cons list, protocols, and a discussion of implementing applications.

Filling the gap in next generation sequencing literature, Next Generation Sequencing: A Primer provides needed instruction for early researchers, students and researchers outside of their expertise across the biological and biomedical sciences.
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Product details

  • Paperback | 240 pages
  • 191 x 235mm
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0124077706
  • 9780124077706

Table of contents

Introduction

Current next generation technologies, clonal approaches, single molecule approaches

Rationale: NGS enables new biological questions

Growth of cells for NGS (using yeast and cultured mammalian cells)

Nucleic acid extraction: DNA and RNA

PCR, gel electrophoresis and quantitative PCR

Overview of diverse types of NGS sequencing libraries.

Preparation of genomic DNA and RNA-seq libraries

Sequencer Operation: overview of diverse platforms

Sequencing on the MiSeq

Data extraction and analysis I: quality filters, trimming, demultiplexing and mapping

Data analysis II: genome viariants- SNPs, CNVs and rearrangements

Data analysis III: RNA seq analysis- gene expression, non-coding sequences, splicing

Data analysis IV: Data visualization and data sharing

Summary and report preparation

Applications of NGS

Perspectives: NEXT next generation technologies
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