Natural Products Isolation

Natural Products Isolation

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This new edition of Natural Products Isolation has been not only significantly updated to reflect recent advances in the field, but has also been expanded with three new chapters on hyphenated techniques, purification by solvent extraction using partition coefficient, and isolation of microbial natural products. The book includes powerful cutting-edge techniques and simple basic techniques that can save time and effort, as well as achieve results equivalent to using some of the more modern and expensive methodologies. The protocols follow the successful Methods in Molecular Biology(t) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.show more

Product details

  • Hardback | 528 pages
  • 157.5 x 231.1 x 33mm | 929.88g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed.
  • 1, black & white illustrations
  • 1588294471
  • 9781588294470
  • 2,461,186

Review quote

"... offers extensive help in the selection of a suitable methodology...an excellent aid...closes a painfully evident gap in the selection of monographs on natural products chemistry...warmly recommended to anyone interested in the isolation of natural products."-Angewandte Chemie "...a very good general reference that concentrates a considerable amount of useful practical information...well worth considering as a reference for graduate students or other researchers who might need to familiarize themselves with the intricacies of the subject."-Journal of Natural Products "...contains a wealth of practical information, hints, and useful points...an asset in any laboratory where natural products isolation is performed." -Journal of Medicinal Chemistry "...a very good general reference that concentrates a considerable amount of useful practical information." -Pathology "...excellent and comprehensive...wholeheartedly recommend[ed]...as a comprehensive handbook for solving problems in daily research." -Drug Discovery Todayshow more

Back cover copy

Building on the high reputation of the first edition of Natural Products Isolation, the authors have in this second edition not only significantly updated all the techniques to reflect recent advances in the field, but also added three new chapters on hyphenated techniques, purification by solvent extraction using partition coefficient, and isolation of microbial natural products. These readily reproducible methods make it possible for even the novice to extract, prepare, and isolate natural products from given source materials. The book includes not only powerful cutting-edge techniques, but also simple basic techniques that can save time and effort, as well as achieve results equivalent to using some of the more modern and expensive methodologies. The protocols follow the successful Methods in Biotechnology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Natural Products Isolation, Second Edition, offers the aspiring natural products researcher all the information needed to attain mastery of essential methods and the more experienced investigator a useful reference guide to all the available techniques.show more

Table of contents

Natural Product Isolation: An Overview Satyajit D. Sarker, Zahid Latif, and Alexander I. Gray Initial and Bulk Extraction Veronique Seidel Supercritical Fluid Extraction Lutfun Nahar and Satyajit D. Sarker An Introduction to Planar Chromatography Simon Gibbons Isolation of Natural Products by Low-Pressure Column Chromatography Raymond G. Reid and Satyajit D. Sarker Isolation by Ion-Exchange Methods David G. Durham Separation by High-Speed Countercurrent Chromatography James B. McAlpine and Patrick Morris Isolation by Preparative High-Performance Liquid Chromatography Zahid Latif Hyphenated Techniques Satyajit D. Sarker and Lutfun Nahar Purification by Solvent Extraction Using Partition Coefficient Hideaki Otsuka Crystallization in Final Stages of Purification Alastair J. Florence, Norman Shankland, and Andrea Johnston Dereplication and Partial Identification of Compounds Laurence Dinan Extraction of Plant Secondary Metabolites William P. Jones and A. Douglas Kinghorn Isolation of Marine Natural Products Wael E. Houssen and Marcel Jaspars Isolation of Microbial Natural Products Russell A. Barrow Purification of Water-Soluble Natural Products Yuzuru Shimizu and Bo Li Scale-Up of Natural Product Isolation Steven M. Martin, David A. Kau, and Stephen K. Wrigley Follow-Up of Natural Product Isolation Richard J. P. Cannell Indexshow more

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