Monographs of the Rockefeller Institute for Medical Research

Monographs of the Rockefeller Institute for Medical Research

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This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1918 edition. Excerpt: ...208, 117.) the infusion cultures showed a mixture of bacteria, among which were spore-bearing bacilli which were morphologically like Bacillus botulinus. The odor of the cultures was characteristic. Portions of the unfiltered and also of the filtered infusion produced the typical syruptoms and thrombosis in animals. There was considerable difliculty in obtaining the club-shaped bacilli in pure culture. Repeated attempts at plating were unsuccessful as Bacillus subtilis grew so much more rapidly that it overgrew the plates. Strain III was ultimately recovered from the spleen of a rabbit which had received an intravenous injection of the mixture of the bacilli, but Strain IV was only obtained in pure culture after a large series of progressive dilutions had been made in deep glucose agar. In both instances the organism proved to be a virulent strain of Bacillus botulinus. Strain V was not contaminated but was obtained in pure culture from the can of beans. The recorded descriptions of the morphology and the cultural characteristics of Bacillus botulinus are based upon a study of the Ellezelles strain (van Ermengem, 34, 86) and of the Darmstadt strain (Landmann, 58). A comparative study of these two strains was recorded by Leuchs (114), and an investigation of these and one other strain was made by von Hibler (116)., The bacilli are large, 4 to 6 by 0.9 to 1.2 microns, have slightly rounded ends, and may arrange themselves in pairs, end to end, or, in unfavorable environment, in long chains. When grown under favorable conditions they form oval spores which are usually situated in the ends of the bacilli, giving them a'club-shaped appearance, but they may be placed in the center, producing spindles (Fig. 1). The bacilli are slightly...show more

Product details

  • Paperback | 48 pages
  • 189 x 246 x 3mm | 104g
  • Rarebooksclub.com
  • United States
  • English
  • black & white illustrations
  • 1236872819
  • 9781236872814