Molecular Motors
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Molecular Motors : Methods and Protocols

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Description

Molecular motor proteins produce force for movement in an incredibly wide variety of cellular processes. This volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family. In addition, it describes techniques directed at motors that fall outside of the three characterized families: dynamin and F1ATPase.
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Product details

  • Hardback | 252 pages
  • 149.86 x 231.14 x 22.86mm | 521.63g
  • Totowa, NJ, United States
  • English
  • 2007 ed.
  • 37 Illustrations, black and white; XII, 252 p. 37 illus.
  • 1588296652
  • 9781588296658

Back cover copy

Molecular motor proteins comprise three protein superfamilies: kinesins, dyneins and myosins. Together, these proteins produce force for movement in an incredibly wide variety of cellular processes including vesicular transport, cell division, nuclear migration, muscle contraction, mechanochemical transduction, among others. In addition, molecular motors are expressed in both simple eukaryotes and in a tissue specific manner in higher cells. This methods volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family. In addition, it describes techniques directed at motors that fall outside of the 3 characterized families (i.e. dynamin and F1ATPase). This volume of the Methods in Molecular Biology series is an essential reference for cell biologists examining the function of motor proteins in a range of model organisms using both in vitro and in vivo techniques. This volume is a valuble source for biochemists and molecular biologists as well.
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Table of contents

Contents

Preface
Contributors

CH. 1 Equilibrium Binding of Proteins to F-Actin, Joseph M. Chalovich
CH. 2 Analysis of Calcium/Calmodulin Regulation of a Plant Kinesin
Using Cosedimentation and ATPase Assays, Anireddy S.N. Reddy
CH. 3 In Vitro and In Vivo Analysis of Microtubule Destabilizing
Kinesins, Jason Stumpff, Jeremy Cooper, Sarah Domnitz, Ayana Moore,
Kathleen Rankin, Mike Wagenbach and Linda Wordeman
CH. 4 Approaches to Kinesin-1 Phosphorylation, Gerardo Morfini, Gustavo
Pigino, and Scott T. Brady
CH. 5 Protein Modification to Probe Intra-dynein Interactions and In
Vivo Redox State, Ken-ichi Wakabayashi, Miho Sakato and Stephen M.
King
CH. 6 Methods to Study the Interactions of the Dynein Light Chains and
Intermediate Chains, Kevin W.-H. Lo and K. Kevin Pfister
CH. 7 Identification of Motor Protein Cargo by Yeast 2-Hybrid And
Affinity Approaches, Yuguo Zhang, Rong Wang, Holly Jefferson, and Ann
O. Sperry
CH. 8 In Situ Binding Assay to Measure Myosin-1c Interactions with
Hair-Cell Proteins, Kelli R. Phillips and Janet L. Cyr
CH. 9 Ultrastructural Analysis of Kinesin-related Motor Proteins During
Spermatogenesis, Wan-Xi Yang
CH. 10 In Vitro Motility System to Study the Role of Motor Proteins in
Receptor-Ligand Sorting, John W. Murray and Allan W. Wolkoff
CH. 11 Enrichment and Disassembly of Ectoplasmic Specializations in the
Rat Testis, Julian A. Guttman, Kuljeet S.Vaid and A. Wayne Vogl
CH. 12 Single-Molecule Observation of Rotation of F1-ATPase through
Micro Beads, Takayuki Nishizaka, Kana Mizutani and Tomoko Masaike
CH. 13 The use of FRET in the Analysis of Motor Protein Structure,
Andre Kasprzak
CH. 14 Structure Determination of the Motor Domain of Yeast Kinesin
Kar3 by X-ray Crystallography, Hee-Won Park
CH. 15 High Resolution Structural Analysis of the Kinesin-Microtubule
Complex by Electron Cryo-Microscopy, Keiko Hirose and LindaA. Amos
CH. 16 Chemical-Genetic Inhibition of Sensitized Mutant Unconventional
Myosins, Ryan L. Karcher, D. William Provance, Jr., Peter G. Gillespie,
and John A. Mercer
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Review quote

From the reviews:


"Within this book's 16 chapters, readers will find a rich source of not only step-by-step protocols and useful lists of the chemicals, reagents, and equipment needed to perform them, but also useful troubleshooting tips and experimental notes that can assist in their successful execution. ... the book is a treasure of data, facts, protocols, and references that nearly every cell biologist and biochemist with an interest in molecular motors will cherish." (Tzvi Tzfira, The Quarterly Review of Biology, Vol. 83, 2008)
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