Molecular Microbial Ecology Manual: Supplement 1
This manual provides general information about molecular techniques relevant to molecular ecological research. Attention is paid to the use of molecular methods for studying the fate of genetically modified and native microorganisms in the environment. The book is intended for postgraduate students and research scientists in the plant and soils sciences and plant molecular biology. The first supplement extends the sections published in the basic work.
- Loose-leaf | 74 pages
- 160.02 x 238.76 x 12.7mm | 476.27g
- 01 Jul 1997
- Kluwer Academic Publishers
- United States
Table of contents
Supplement 1: 1.1.1. Extraction of microbial DNA from aquatic sources: Marine environments; J.H. Paul. 1.2.1. Extraction of microbial RNA from aquatic sources: Marine environments; S.L. Pichard, J.H. Paul. 1.3.2. Direct extraction of microbial DNA from soils and sediments; W.H. Johnston, et al. 1.4.1. Detection of microbial genes from the rhizosphere by magnetic capture hybridization and subsequent amplification of target genes by PCR; C. Jacobson. 2.1.1. Quantification of nucleic acids; V. Torsvik. 2.1.2. Parameters of nucleic acid hybridization experiments; C.N. Nakatsu, L.J. Forney. 2.8.2. Soil; E. Baath. - Basic Work: Extraction of Microbial DNA from Sewage and Manure; K. Smalla. Cell Extraction Method; V. Torsvik. Polymerase Chain Reaction (pcr) Analysis of Soil Microbial DNA; J.D. van Elsas, A.C. Wolters. Extraction and pcr Amplification of DNA from the Rhizoplane; P.A. Bramwell, et al. Extraction of Microbial DNA from the Phylloplane; P.A. Bramwell, et al. Extraction of Ribosomal DNA from Microbial Cultures; E. Stackebrandt, N. Ward. In situ Identification of Micro-organisms by Whole Cell Hybridization with RNA-Targeted Nucleic Acid Probes; R.I. Amann. Immunofluorescence Colony-Staining (IFC); J.W.L. van Vuurde, J.M. van der Wolf. Fluorescent Staining of Microbes for Total Direct Counts; J. Bloem. Natural Transformation in Aquatic Environments; J.H. Paul, H.G. Williams. Phage Ecology and Genetic Exchange in Soil; P.R. Herron. Design of Microcosms to Provide Data Reflecting Field Trials of GEMs; M.A. Hood, R. J. Seidler. Methods for Extracting DNA from Microbial Mats and Cultivated Micro-organisms: High Molecular Weight DNA from French Press Lysis; M.M. Bateson, D.M. Ward. Bacterial Community Fingerprinting of Amplified 16S and 16-23S Ribosomal DNA Gene Sequences and Restriction Endonuclease Analysis (ARDRA); A.A. Massol-Deya, et al. Detection of Gene Transfer in the Environment: Conjugation in Soil; E. Smit, J.D. van Elsas. Direct and Simultaneous Extraction of DNA and RNA from Soil; S. Selenska-Pobell. Sequence Databases; W. Ludwig. Extraction of Microbial Community DNA from Soils; J.D. van Elsas, K. Malla. Investigation of Fungal Phylogeny on the Basis of Small Ribosomal Subunit RNA Sequences; Y. van de Peer, R. de Wachter. Biodegradation Genes as Marker Genes in Microbial Ecosystems; B.M. Applegate, et al. Preparation of Radioactive Probes; M. Cunningham. Detection of Nucleic Acids by Chemiluminescence; M. Cunningham, et al. Gel Purification of Soil DNA Extracts; D.D. Myrold, et al. Detection of Microbial DNA Sequences by Colony Hybridization; P.R. Hirsch. Partial and Complete 16s RDNA Sequences, their Use in Generation of 16s RDNA Phylogenetic Trees and their Implications in Molecular Ecological Studies; E. Stackebrandt, F.A. Rainey. Extraction of Microbial DNA from Aquatic Sources: Freshwater; R.W. Pickup, et al. Slide Immunoenzymatic Assay (SIA); E.C. de Macario, A.J.L. Macario. (Part contents).