Mitochondria: Volume 80

Mitochondria: Volume 80

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This book provides an update on the step-by-step "how to" methods for the study mitochondrial structure, function, and biogenesis contained in the successful first edition. As in the previous edition, the biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls from each method.
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Product details

  • Hardback | 944 pages
  • 191 x 235 x 38.1mm | 1,740g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 2nd edition
  • Approx. 120 illustrations; Illustrations, unspecified
  • 0125441738
  • 9780125441735
  • 2,116,741

Table of contents

Isolation and Subfractionation of Mitochondria
1. Isolation and Subfractionation of Mitochondria in Animal Cells
2. Purification and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae
3. Isolation and Subfractionation of Mitochondria from Plants

Biochemical Assays of Mitochondrial Activity
4. Biochemical Assays of Respiratory Chain Complex Activity
5. Polarographic Assays of Respiratory Chain Complex Activity
6. Optical Imaging Techniques (Histochemical, Immunohistochemical and In Situ Hybridization Staining Methods) to Visualize Mitochondria
7. Assay of Mitochondrial ATP Synthesis in Animal Cells and Tissues
8. Measurement of the Ratio of Lactate to Pyruvate in Skin Fibroblast Cultures
9. Assays of Fatty Acid fO-Oxidation Activity
10. Biochemical Assays for Mitochondrial Activity: Assays of TCA Cycle Enzymes and PDHc
11. Assays of Cardiolipin Levels
12. Measurement of VDAC Permeability in Intact Mitochondria and in Reconstituted Systems
13. Methods for Studying Fe/S Cluster Protein Maturation in Yeast Mitochondria

Assays for Mitochondrial Respiratory Activity and Permeability in Living Cells
14. Imaging of Mitochondrial Polarization and Depolarization with Cationic Fluorophores
15. Biosensors for the Detection of Calcium and pH
16. Measurement of Membrane Permeability and the Permeability Transition of Mitochondria
17. Luciferase Expression for ATP Imaging: Application to Cardiac Myocytes

Oxidative Stress Measurements
18. Measurement of Reactive Oxygen Species in Cells and Mitochondria
19. Measurements of the Antioxidant Enzyme Activities of Superoxide Dismutase, Catalase, and Glutathione Peroxidase
20. Methods for Measuring the Regulation of Respiration by Nitric Oxide
21. Methods for Determining the Modification of Protein Thiols by Reactive Lipids

Mitochondrial Genes and Gene Expression
22. Detection of Mutations in mtDNA
23. Diagnostic Assays for Defects in mtDNA Replication and Transcription in Yeast and Humans
24. Microdissection and Analytical PCR for the Investigation of mtDNA Lesions
25. Transmitochondrial Technology in Animal Cells
26. Genetic Transformation of Saccharomyces cerevisiae and Chlamydomonas Reinhardtii Mitochondria
27. Generation of Transmitochondrial Mice: Development of Xenomitochondrial Mice to Model Neurodegenerative Diseases
28. In vivo and In organello Analysis of Mitochondrial Translation
Assays for Mitochondrial Morphology and Motility
29. Visualization of Mitochondria in Budding Yeast
30. Visualization and Quantification of Mitochondrial Dynamics in Living Animal Cells
31. Cell-Free Assays for Mitochodnrial-Cytoskeletal Interactions
32. Assays to Measure Mitochondrial Fusion In Vitro and the GTPase Activity of Mitochondrial Fission Proteins

Methods to Determine Protein Localization to Mitochondria
33. Electrophoretic Methods to Isolate Protein Complexes from Mitochondria
34. Analysis of Protein-Protein Interactions in Mitochondria
35. Analysis and Prediction of Mitochondrial Targeting Signals
36. Import of Proteins into Mitochondria

Appendix 1: Basic Properties of Mitochondria
Appendix 2: Direct and Indirect Inhibitors of Mitochondrial ATP Synthesis
Appendix 3: Linearized Maps of Mitochondrial Genomes from Representative Organisms
Appendix 4: Mitochondrial Genetic Codes in Various Organisms
Appendix 5: Gene Products Present in Mitochondria of Yeast and Animal Cells
Appendix 6: Changes in the Mitochondrial Transcriptome and Proteome Under Various Stresses and Growth Conditions
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