Microtubules: in vivo: Volume 97

Microtubules: in vivo: Volume 97

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Microtubules: in vivo includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.
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Product details

  • Hardback | 552 pages
  • 185.42 x 236.22 x 25.4mm | 1,043.26g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 97th edition
  • 0123813492
  • 9780123813497

Table of contents

Microtubule dynamics at the cell cortex probed by TIRF microscopy
Ilya Grigoriev and Anna Akhmanova

Microscopy methods for the study of centriole biogenesis and function in Drosophila
Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica Bettencourt-Dias

Quality control in single molecule studies of kinesins and microtubule-associated proteins
Gary J. Brouhard

Imaging cilia in zebrafish
Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and Rebecca D. Burdine

Automatic Identification of Microtubules in Cellular Electron Tomography
Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman

Imaging and analysis of the microtubule cytoskeleton in Giardia
Scott C. Dawson and Susan A. House

Kinetochore-microtubule dynamics and attachment stability
Jennifer G. DeLuca

Assessment of mitotic spindle phenotypes in Drosophila S2 cells
Gohta Goshima

Live cell imaging techniques for analyses of microtubules in Dictyostelium
Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Graf

Microtubule dynamics in dendritic spines
Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad

Analysis of Microtubules in Budding Yeast
Alexander Rauch, Elena Nazarova and Jackie Vogel

Determination of Microtubule Dynamic Instability in Living Cells
Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan

Modeling microtubule-mediated forces and centrosome positioning in Caenorhabditis elegans embryos
Akatsuki Kimura1 and Shuichi Onami

Cryo electron tomography of cellular microtubules
Roman I. Koning

Microtubule dynamics in plant cells
Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and Clive W. Lloyd

Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, architecture and function
Sara Moutinho-Pereira, Irina Matos and Helder Maiato

Protein micropatterns: a direct printing protocol using deep UVs
Ammar Azioune, Nicolas Carpi, Manuel Thery and Matthieu Piel

Optical trapping and laser ablation of microtubules in fission yeast
Nicola Maghelli and Iva M. Tolic-Norrelykke

Melanophores for Microtubule dynamics and motility assays
Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov

New and old reagents for fluorescent-protein tagging of microtubules in fission yeast: experimental and critical evaluation
Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin

Imaging of mitotic spindle dynamics in C. elegans embryos
Mika Toya, Yumi Iida and Asako Sugimoto

A fast microfluidic temperature control device for studying microtubule dynamics in fission yeast
Guilhem Velve-Casquillas, Judite Costa, Frederique Carlier-Grynkorn , Adeline Mayeux and Phong T. Tran

Microtubule-dependent spatial organization of mitochondria in fission yeast
Maitreyi Das, Stephan Chiron and Fulvia Verde

Photoactivatable GFP-Tubulin
U. Serdar Tulu*, Nick P. Ferenz

The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics
Claire E. Walczak, Rania S. Rizk and Sidney L. Shaw

Analysis of microtubule polymerization dynamics in live cells

Sarah Gierke, Praveen Kumar, and Torsten Wittmann
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