Microtubules, in vitro: Volume 115

Microtubules, in vitro: Volume 115

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There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This volume (complemented by the forthcoming companion volume by Cassimeris and Tran) brings together current research and protocols in the field of microtoubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors.
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Product details

  • Hardback | 699 pages
  • 182.88 x 259.08 x 33.02mm | 1,383.45g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0123748151
  • 9780123748157

Table of contents

Section1: Isolation, Biochemistry and Characterization of Antibodies and Isotypes.

Preparation of Microtubule Protein and Purified Tubulin from Bovine Brain by Cycles of Assembly and Disassembly and Phosphocellulose Chromatography
Herbert P. Miller and Leslie Wilson

Measurement Of Ligand Binding To Tubulin By Sulfhydryl Reactivity
Adrian Begaye and Dan L Sackett

Isolating Tubulin From Nonneural Sources
Dan L. Sackett, Karl A. Werbovetz and Naomi S. Morrissette

Characterization of Anti-b -Tubulin Antibodies
Anthony J. Spano* and Anthony Frankfurter

Expression profiling of tubulin isotypes and microtubule interacting proteins using real-time polymerase chain reaction
Sharon Lobert, Laree Hiser and John J. Correia

Nondenaturing Electrophoresis As A Tool To Investigate Tubulin Complexes
Monica Lopez-Fanarraga, Gerardo Carranza, Raquel Castano, Sofia Nolasco, J. Avila and J.C. Zabala

Mass spectrometry analysis of c-terminal posttranslational Modifications of tubulins
Redeker, Virginie

Methods in Tubulin Proteomics
Leah M. Miller, Hui Xiao, Berta Burd, Susan Band Horwitz, Ruth Hogue Angeletti, Pascal Verdier-Pinard

Section 2: Microtubule Structure and Dynamics

Cryo-EM Studies of Microtubule Structural Intermediates and Kinetochore-Microtubule Interactions
Eva Nogales, Vincent H. Ramey and Hong-Wei Wang

High resolution imaging of microtubules and cytoskeleton structures by Atomic Force Microscopy
Loic Hamon, Patrick A. Curmi, David Pastre

Holly V. Goodson and Ivan V. Gregoretti
Using computational modeling to understand microtubule dynamics: a primer for cell biologists

Analysis of Dynamic Instability of Steady State Microtubules In Vitro by Video-Enhanced Differential Interference Contrast Microscopy with an Appendix by Emin Oroudjev
Mythili Yenjerla, Manu Lopus and Leslie Wilson

Nanometer-resolution microtubule polymerization assays using optical tweezers and microfabricated barriers
Blake D. Charlebois, Henry T. Schek III, Alan J. Hunt

Microtubule Dynamics Reconstituted in-vitro and Imaged y Single-Molecule Fluorescence Microscopy
Christopher Gell, Volker Bormuth, Gary J. Brouhard, Daniel N. Cohen, Stefan Diez, Claire T. Friel, Jonne Helenius, Bert Nitzsche, Heike Petzold, Jan Ribbe, Erik Schaffer, Jeffrey H. Stear, Anastasiya Trushko, Vladimir Varga, Per O. Widlund, Marija Zanic, and Jonathon Howard

Studying kinesin motors by optical 3D-nanometry in gliding motility assays
Bert Nitzsche, Volker Bormuth, Corina Brauer, Jonathon Howard, Leonid Ionov, Jacob Kerssemakers, Till Korten, Cecile Leduc, Felix Ruhnow, Stefan Diez

Section 3: Drugs

Analysis of tubulin oligomers by Analytical Ultracentrifugation.
J.J. Correia

Determination of Drug Binding to Microtubules in vitro
Jennifer A. Smith and Mary Ann Jordan

Fluorescence Spectroscopic Methods to Analyze Drug-Tubulin Interactions
Bhabatarak Bhattacharyya, Sonia Kapoor and Dulal Panda

A Tubulin polymerization micro-assay used to compare ligand efficacy
Ashley Davis, Sarah Martinez, Daniel Nelson and Kim Middleton.

Fluorescent Taxoid Probes for Microtubule Research
Isabel Barasoain, J. Fernando Diaz, and Jose M. Andreu

The binding of vinca domain agents to tubulin: structural and biochemical studies
Anthony Cormie, Marcel Knossow, Chunguang Wang and Benoit Gigant

Section 4: Interactions with Motors and MAPs

Probing Interactions of Tubulin with Small Molecules, Peptides and Protein Fragments by Solution Nuclear Magnetic Resonance
Marie-Jeanne Clement, Philippe Savarin, Elisabeth Adjadj, Andre Sobel, Flavio Toma, Patrick A. Curmi

Microtubule and MAPs: thermodynamics of complex formation by AUC, ITC, fluorescence and NMR
Francois Devred, Pascale Barbier, Daniel Lafitte, Isabelle Landrieu, Guy Lippens and Vincent Peyrot

Quantitative Analysis of MAP Mediated Regulation of Microtubule Dynamic Instability In Vitro - Focus on Tau
Erkan Kiris, Donovan Ventimiglia and Stuart C. Feinstein

Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopy (FPM)
Hernando Sosa, Ana B. Asenjo and Erwin J.G. Peterman

Multiple color single molecule TIRF imaging and tracking of MAPs and motors
Jennifer L. Ross and Ram Dixit

Dixit and Ross - final submitted 11/4 & lost - resubmitted 1/8/10
Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor and +TIP dynamics
Peter Bieling, Ivo A. Telley, Christian Hentrich, Jacob Piehler and Thomas Surrey

Section 5: Functional Extracts and Force Measurements

Quantitative Characterization of filament dynamics by single-molecule lifetime measurements.
Leonid A. Mirny and Daniel J. Needleman

Extracting the mechanical properties of microtubules from thermal fluctuation measurements on an attached tracer particle.
Katja M. Taute, Francesco Pampaloni, Ernst-Ludwig Florin

In vitro assays to study force generation at dynamic microtubule ends
Liedewij Laan and Marileen Dogterom

Reconstitution and functional analysis of kinetochore subcomplexes
Daniel R. Gestaut, Jeremy Cooper, Charles L. Asbury, Trisha N. Davis and Linda Wordeman

In vitro assays to study the tracking of shortening microtubule ends and to measure associated forces

Ekaterina L. Grishchuk and Fazly I. Ataullakhanov
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