Microtubules, in vitro: Volume 115

Microtubules, in vitro: Volume 115

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There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This 2e brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors.
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Product details

  • Hardback | 440 pages
  • 192 x 238 x 24mm | 859.99g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 2nd edition
  • 0124077579
  • 9780124077577

Table of contents

Isolation of Antarctic fish tubulin and MAPs

Making, isolating and characterizing tubulin alpha antibodies

Techniques for labeling tubulin and drugs

Single-particle 3D reconstruction methods for decorated and undecorated microtubules

A new microtubule gliding assay and analysis of microtubule persistence length

Characterization of gamma tubulin

Biochemical and Structural Studies of the doublecortin family of MAPs

Detection and quantification of microtubule detachment from centrosomes and spindle poles

Regulation of tubulin expression by micro RNAs: Implications for Drug Resistance

A portable magnetic tweezers device to visualize three-dimensional deformation of reconstituted microtubule networks

Mitochondrial/microtubule interactions - mmbp1

Going Solo: Measuring the motions of microtubules with an in vitro assay for TIRF microscopy

Analysis of Microtubules and Motors in Isolated Axoplasm from the Squid Giant Axon

Analysis of drug binding to microtubules and steady state microtubule growth/treadmilling by use of a sedimentation assay in vitro

Specific recombinant antibody technology for the study of microtubule dynamics and regulatory proteins

Tubulin-specific Chaperones: Components of a Molecular Machine that Assembles the aB ï  Heterodimer

G-protein tubulin interactions

Katanin, a microtubule severing enzyme: expression, purification, and biophysical characterization

Measuring tubulin polymerization in vitro - comparison of methods and possible artifacts

Imaging microtubules in living plant cells

How to use which antibody known in the field, what to expect from this antibody and how semi-quantitative data can be reliably obtained with antibody staining

Preparation of bacterial tubulin BtubA/B and constructs bearing eukaryotic tubulin sequences

Microtubule-associated proteins and tubulin interaction by isothermal titration calorimetry


Characterization of the mechanism of action of the covalent-binding microtubule-stabilizing agent zampanolide

Studying kinetochore-fibre ultrastructure using correlative light-electron microscopy

Fluorescence-based Assays for Microtubule Structure

Yeast Tubulin-structure-function analysis

Microtubule Binding SIm to simulate binding of proteins to polymers under different binding models
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