Microsome

Microsome

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Description

Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. In cell biology, microsomes are vesicle-like artifacts formed from the endoplasmic reticulum (ER) when eukaryotic cells are broken-up in the laboratory; by definition, microsomes are not ordinarily present in living cells. Microsomes can be concentrated and separated from other cellular debris by differential centrifugation. Unbroken cells, nuclei, and mitochondria sediment out at 10,000g, whereas soluble enzymes and fragmented ER, which contains cytochrome P450, remain in solution (g is the Earth's gravitational acceleration). At 100,000g, achieved by faster centrifuge rotation, ER sediments out of solution as a pellet but the soluble enzymes remain in the supernatant. In this way, cytochrome P450 in microsomes is concentrated and isolated. Microsomes have a reddish brown color, due to the presence of the iron-containing co-factor, heme (haem), in the P450s. P450s are highly abundant in livers of rats, mice and humans.show more

Product details

  • Paperback | 72 pages
  • 152 x 229 x 4mm | 118g
  • Chrono Press
  • United States
  • English
  • 6135738925
  • 9786135738926