Methods in Toxicology: v.3

Methods in Toxicology: v.3

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Description

Efforts to understand the adverse effects of reproductive toxicants must address a series of precisely times interactions, not only among the organs involved (brain, pituitary, and ovary) but also within those organs in the female, pregnancy may vary the primary effect, so events that occur in both the pregnant and non-pregnant animal must be assessed. This requires the use of a broad array of in vivo and in vitro techniques. This book brings together for the first time in a single volume many methods for assessing the behavioral, hormonal, and cellular changes that occur at all levels during exposure to reproductive toxicants. Each chapter contains not only a detailed, step-by-step protocol, but also the reasons for the key steps - as well as possible variations - where applicable. For easy reference, the book is organized first by pregnancy state, then by tissue response, then by cells within that tissue. These are all tied together by an introduction that puts each method into perspective within the field and provides ways of integrating the different methods to generate a complete understanding of the effects a toxicant create.
The intent is to demystify the complexities of reproductive toxicology so that more investigators will address the area and will, in turn, produce a better understanding of the sites and modes of action of reproductive toxicants.
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Product details

  • Spiral bound | 404 pages
  • 171 x 236 x 23mm | 631g
  • Academic Press Inc
  • San Diego, United States
  • English
  • subject index
  • 0124612105
  • 9780124612105

Table of contents

Use of perifusion to evaluate hormonal release in vitro and rat pituitary and hypothalamic tissue, J.M. Goldman and R.L.Cooper; measuring sexual behavior in the female rat, R.L. Cooper et al; monitoring of the estrous cycle in the laboratory rodent by vaginal lavage, R.L. Cooper et al; assessment of toxicity to the ovary using follicle quantitation and morphometrics, D.R. Plowchalk et al; ovarian target cell toxicity, B.J. Davis; assessment of toxicant-induced alterations in the luteinizing hormone control of ovulation in the rat, J.M. Goldman and R.L. Cooper; strategies and methods for evaluating the functional capacity of oocytes and zygotes in vitro, S.D. Perreault and S. Jeffay; recovery and morphological evaluation of oocytes, zygotes and preimplantation embryos, S.D. Perreault and B.A. Mattson; mouse oocytes and embryo culture, M.J. O'Brien et al; isolation and culture of ovarian follicles from hamster mouse, and pig, G.S. Greenwald et al; the in vitro perfused rat ovary, M. Brannstrom; isolated perfused rabbit ovary preparation, A.M. Dharmarajan et al; ovarian perifusion culture - a tool to assess ovarian toxicity, J.J. Peluso and A. Pappalarda; assessment of implantation in the rat, A.M. Cummings; pseudopregnancy and the decidual cell response in the rat, A.M. Cummings; perfusion of the placenta, R. Miller; trophoblast culture, R. Miller; in vitro exposure of preimplantation embryos to potential toxicants with subsequent embryo transfer and evaluation, N.L. Bossert; isolation and culture of rat granulosa cell cultures, K.A. Treinen; human granulosa and granulosa luteal cell in culture, D.A. Magoffin; porcine granulosa and theca cell culture - bioassay applications for factors modulating cell proliferation and differentiation, J.V. May; rat thecal-interstitial cells in culture, D.A. Magoffin; human theca interna cells in long-term culture, J.M. McAllister and E.R. Simpson; dispersion, separation, and culture of the different cell populations of the rat, corpus luteum, S. Nelson and G. Gibori; isolation and culture of fully differentiated bovine luteal cells, J.L. Pate; isolation and culture of mouse uterine, epithelial and stroma cells, K.A. Ross et al.
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