Methods in Neurosciences: Receptors - Model Systems and Specific Receptors v. 11

Methods in Neurosciences: Receptors - Model Systems and Specific Receptors v. 11

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Receptors initiate the means by which cellular regulators exert their actions on target cells. Because of the central role of cell-cell communication and signal transduction, receptors are of intrinsic interest to neuroscientists. Receptor studies utilize both traditional methods of analysis and modern molecular techniques. This volume examines comprehensive protocols for "model" receptor techniques (radioligand binding, solubilization and purification). It also describes the methodology for the following receptors: bombesin/GRP, cholecystokinin, endothelin, fast nerve growth factor and gastrin-releasing peptide, insulin, insulin-like growth factors, interleukin 1, melanotropin, melatonin, natriuretic hormone, neurokinin, neurokinin, neurotensin, PACAP, pancreatic polypeptide, serotonin, steroids, transferrin and vasoactive peptides.
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Product details

  • Hardback | 428 pages
  • Academic Press Inc
  • San Diego, United States
  • English
  • chapter references, subject index
  • 0121852717
  • 9780121852719

Table of contents

Steroid receptors in the central nervous system, L.M. Freeman and S.M. Breedlove; membrane receptor for glucocorticoids in mammalian neurons, Y. Cehn, H. Fu, et al; interleukin-1 receptors in the brain-endocrine-immune axis, T. Takao and E.B. De Souza; vasoactive peptides and their receptors, D. Regoli, A. Cadieux, et al; melanotropin receptors of the brain, J.B. Tatro; melatonin binding sites, E.L. Bittman; transferrin receptors in the central nervous system, B. Giometto, P. Gallo, et al; binding sites of pituitary adenylate cyclase activating polypeptide (PACAP) in human brain, D.M. Smith and S.R. Bloom; role of cholecystokinin type B receptor in nociception studied with peptide agonists and antagonists, Z. Wiesenfeld Hallin and X.J. Xu; cholecystokinin receptors - radioligand binding and affinity labelling, M.P. Lutz and L.J. Miller; endothelin receptors in neural systems, P.G. Lysko and P. Nambi; natriuretic peptide receptors, V.T.F. Yeung, C.S. Cockram, et al; structural and functional identification of insulin-like growth factor receptors, S. Gammeltoft; methods for studying central serotonergic receptors, P.M. Whitaker-Azmitia; analysis of serotonin 5-HT1A receptors in primary neuronal culture, B.C. Swarzenski, A.M. Benz, et al; characterization of the bombesin/GRP receptor in swiss 3T3 fibroblasts, I. Zachary, J. Sinnett-Smith, et al; receptors for bombesin-like peptides in the rat central nervous system, E.E. Ladenheim, T.H. Moran, et al; retinal insulin receptors, S.A. Rosenzweig; pancreatic polypeptide receptors in rat brain, D.C. Whitcomb, S.R. Vigna, et al; neurotensin receptors in primary culture of neurons, J. Mazella, J. Chabry, et al; analysis of the structure and function of the fast nerve growth factor receptor, A.A. Welcher; solubilization and physicochemical characterization of 5-HT3 receptor binding sites, M.C. Miquel, M.B. Emerit, et al; solubilization and characterization of the receptor for gastrin-releasing peptide, R. Kris, D. Cirillo, et al; assays for pituitary adenylate cyclase activating peptide (PACAP) receptors coupled to adenylate cyclase - comparison between rat brain and human neuroblastoma cells, J. Christophe, A. Vandermeers, et al.
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