Methods in Neurosciences: Photoreceptor Cells v. 15

Methods in Neurosciences: Photoreceptor Cells v. 15

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Advances in the study of photoreceptor cells, which have proceeded at a rapid pace during the past decade, have increased our understanding of cell functions. This volume presents up-to-date biochemical, cell and structural biological, and molecular biological techniques needed for the study of photoreceptor cells. Methods presented for easy adaptation to new systems include: molecular biological techniques related to retinal research; preparative methods for proteins in the visual transduction system; and microscopy methods adapted specifically for the retina.
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Product details

  • Hardback | 316 pages
  • 196.85 x 230 x 25.4mm | 981g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0121852792
  • 9780121852795

Table of contents

Part 1 Structure of photoreceptor cells: light and electron microscopy of vertebrate photoreceptors, S.K. Fisher, et al; rapid freezing and subsequent preparation methods in retinal cell biology, J. Usukura; preparation of retinas for studying photoreceptors with the confocal microscope, B. Matsumoto and I.L. Hale. Part 2 Metabolic studies: labelling of retinal components by intraocular injection, P.J. O'Brien; biosynthetic studies with isolated vertebrate retinas, S.J. Fliesler; isolation of post-golgi membranes transporting newly synthesized rhodopsin, D. Deretic and D. Papermaster; electropermeabilized frog rod outer segments, P.D. Calvert, et al. Part 3 Photoreceptor membrane and protein preparation and characterization: preparing rod outer segment membranes, regenerating and determining rhodopsin concentration, J.H. McDowell; isolation and characterization of rod outer segment disk and plasma membranes, R.S. Molday and L.L. Molday; immunological measurement of rhodopsin, G. Adams; preparation and characterization of chicken rod and cone pigments, T. Yoshizawa and Y. Fukada; purification and charactrization of bovine transducin and its subunits, T.D. Ting, et al; purification and characterization of phosducin from bovine retina, R.H. Lee and R.N. Lolley; pH assay of rod outer segment cGMP phosphodiesterase activity, M. W. Kaplan and K. Placzewski; purification of rhodopsin kinase from bovine rod outer segments, K. Palczewski; purification of arrestin from bovine retinas, J. Buczylko and K. Palczewski; preparation and characterization of guanylate cyclase from vertebrate rod photoreceptors, F. Hayashi, et al; calcium binding proteins in the retina, A.S. Polans, et al; simultaneous purification and characterization of the cGMP-gated cation chanel and the na-ca, K-exchanger. Part 4 Molecular biology of photoreceptor cells: construction of directional cDNA libraries from human retinal tissue/cells and their enrichment for specific genes using an efficient subtraction procedure, A. Swaroop; expression of opsin genes in COS cells, D.D. Oprian; synthesis of bovine rhodopsin using recombinant baculovirus, G.L.J. DeCaluwe, et al; transfection of retinoblastoma cells - localization of cis-acting elements for human interstitial retinoid binding protein (IRBP) gene expression, S.L. Fong, et al; use of transgenic mice to study retinal gene expression, D.J. Zack; transgenic rescue of the photoreceptor dysplasia and degeneration in retinal degeneration slow (rds) mutant mice, G.H. Travis and D. Bok; quantitative in situe hybridization of retinal mRNA using nonradioactive antisense synthetic probes, J.G. Flannery, et al; identification of rhodopsin gene mutations using GC-clamped denaturing gradient gel electrophoresis, E.M. Stone, et al.
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