Methods in Neurosciences: Peptidases and Neuropeptide Processing v.23

Methods in Neurosciences: Peptidases and Neuropeptide Processing v.23

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The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are a useful aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. This volume includes a variety of diverse techniques used to identify, characterize and localize peptidases and peptide processing enzymes. The techniques range from classical protein purification, through localization studies, to state-of-the-art molecular biological techniques to examine regulation and expression. Major topics covered include: characterization of enzymes involved in the activation of bioactive peptidases and proteins; methods for characterizing prohormone/neuropeptide processing pathways; identification and characterization of enzymes involved in peptide metabolism; and post-translational processing enzymes.
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Product details

  • Hardback | 402 pages
  • 203 x 248 x 25.4mm | 976g
  • Academic Press Inc
  • San Diego, United States
  • English
  • references, index
  • 0121852938
  • 9780121852931

Table of contents

Part 1 Molecular approaches for the study of intracellular processing enzymes: molecular strategies for identifying processing enzymes, N.G. Seidah; "in situ" hybridization techniques to map processing enzymes, M.K.-H. Schafer and R. Day; analysis of ontogeny of processing enzyme gene expression and regulation, M. Zheng and J.E. Pintar; use of vaccinia virus vectors to study neuropeptide processing, J.K. VanSlyke et al; overexpression of neuropeptide precursors and processing enzymes, I. Lindberg and Y. Zhou; use of antisense RNA to block peptide-processing enzyme expression, R.E. Mains. Part 2 Immunological and biochemical approaches to the study of peptide-processing pathways: combination of high-performance liquid chromatography and radioimmunoassay for characterization of peptide-processing pathways, A.I. Smith and R.A. Lew; development and use of two-site immunometric assays for examining peptide-processing pathways, S.R. Crosby; methods for identification of neuropeptide-processing pathways, P. Cohen et al; immunological and related techniques for studying neurohypophyseal peptide-processing pathways, H. Gainer et al; approaches to assessing ontogeny of processing enzymes, R.G. Allen and J. Stack; measurement, distribution and subcellular localization of peptide-amidating activity, R.A. Lew and A.I. Smith; methods for studying carboxypeptidase, L.D. Fricker; characterization of endothelin-converting enzymes, T.J. Opgenorth et al; "in vivo" approaches for studying peptide processing, A. Shulkes. Part 3 Identification and characterization of extracellular processing enzymes in the central nervous system: identification and characterization of central nervous system peptidase activities, J.R. McDermont and A.M. Gibson; strategies for characterizing, cloning and expressing soluble endopeptidases, M.J. Glucksman and J.L. Roberts; proteolytic processing and amyloid protein precursor of Alzheimer's disease, D.H. Small et al; strategies for measurement of angiotensin and bradykinin peptides and their metabolites in central nervous system and other tissues, D.J. Campbell et al; distribution and roles of endopeptidase 24.11, A.J. Turner and K. Barnes; identification and distribution of endopeptidase 24.16 in the central nervous system, F. Checler et al; autoradiographic techniques to map angiotensin-converting enzyme in brain and other tissues, S.Y. Chai and F.O. Mendelsohn.
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About A.Ian Smith

Edited by A. R. Smith, and Michael P. Conn
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Review quote

"Well-written, clear and detailed... The authors do indeed offer the 'fine detail, tricks and short cuts' not usually found in the written word." TRENDS IN NEUROSCIENCES
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