Part 1 Molecular approaches for the study of intracellular processing enzymes: molecular strategies for identifying processing enzymes, N.G. Seidah; "in situ" hybridization techniques to map processing enzymes, M.K.-H. Schafer and R. Day; analysis of ontogeny of processing enzyme gene expression and regulation, M. Zheng and J.E. Pintar; use of vaccinia virus vectors to study neuropeptide processing, J.K. VanSlyke et al; overexpression of neuropeptide precursors and processing enzymes, I. Lindberg and Y. Zhou; use of antisense RNA to block peptide-processing enzyme expression, R.E. Mains. Part 2 Immunological and biochemical approaches to the study of peptide-processing pathways: combination of high-performance liquid chromatography and radioimmunoassay for characterization of peptide-processing pathways, A.I. Smith and R.A. Lew; development and use of two-site immunometric assays for examining peptide-processing pathways, S.R. Crosby; methods for identification of neuropeptide-processing pathways, P. Cohen et al; immunological and related techniques for studying neurohypophyseal peptide-processing pathways, H. Gainer et al; approaches to assessing ontogeny of processing enzymes, R.G. Allen and J. Stack; measurement, distribution and subcellular localization of peptide-amidating activity, R.A. Lew and A.I. Smith; methods for studying carboxypeptidase, L.D. Fricker; characterization of endothelin-converting enzymes, T.J. Opgenorth et al; "in vivo" approaches for studying peptide processing, A. Shulkes. Part 3 Identification and characterization of extracellular processing enzymes in the central nervous system: identification and characterization of central nervous system peptidase activities, J.R. McDermont and A.M. Gibson; strategies for characterizing, cloning and expressing soluble endopeptidases, M.J. Glucksman and J.L. Roberts; proteolytic processing and amyloid protein precursor of Alzheimer's disease, D.H. Small et al; strategies for measurement of angiotensin and bradykinin peptides and their metabolites in central nervous system and other tissues, D.J. Campbell et al; distribution and roles of endopeptidase 24.11, A.J. Turner and K. Barnes; identification and distribution of endopeptidase 24.16 in the central nervous system, F. Checler et al; autoradiographic techniques to map angiotensin-converting enzyme in brain and other tissues, S.Y. Chai and F.O. Mendelsohn.