Methods in Neurosciences: PCR in Neuroscience v. 26
This book presents important aspects of PCR that enables one to devise simpler routes in solving problems pertinent to the neurosciences. It has been designed to meet the needs of the student and experienced researcher.;Major topics covered include: Direct application of PCR to fresh or frozen clinical specimens (eg. blood and solid tissue); Complete retrieval of novel expressed genes by PCR without screening a library; Quantitation by PCR; and Simple and effective protocols for PCR on archival specimens.
- Paperback | 440 pages
- 203 x 248 x 53mm | 1,053g
- 31 May 1995
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
- index, references
Table of contents
Part 1 Direct PCR on clinical specimens: formamide low-temperature PCR - applications for direct PCR from clinical material, M. Panaccio et al; direct use of blood in PCR, B.-Y. Nordvag et al. Part 2 Application of reverse transcription-mediated PCR: use of PCR for isolation of neuropeptide receptor genes, T.K. Chatterjee et al; lock-docking RACE PCR - strategies and applications, N.D. Borson et al; single-cell cDNA-PCR, B.L. Ziegler et al. Part 3 Gene synthesis by PCR: PCR-mediated synthesis of chimeric molecules, A. Darveau et al; PCR-mediated gene synthesis, K. Jayaraman. Part 4 Quantitative PCR: quantitative PCR - analysis of rare mitochondrial DNA mutations in central nervous system tissues, N.W. Soong and N. Arnheim; PCR-aided transcript titration assay - competitive PCR for evaluation of absolute levels of rare mRNA species, M. Becker-Andre; comparative evaluation of quantitative PCR methods, S. Sur et al. Part 5 Application of PCR in mutation detection: mutational detection by single-strand conformational polymorphism, N. Bardeesy and J. Pelletier; detection of mutation in yeast hsp60 gene by PCR, A. Sanyal and G.S. Getz; comparison of the sensitivity of single-strand conformational polymorphism and heteroduplex methods, D. Glavac and M. Dean; analysis of p53 mutations in human gliomas by RNA single-strand conformational polymorphism, T.M. Cheng et al. Part 6 Generation of probes by PCR: PCR and generation of antisense RNA probes for use in RNase protection assays, H. Yang and P.W. Melera; in vitro transcription of cRNA from PCR-generated DNA fragments, S. Leonard. Part 7 PCR in the context of cloning and constructing libraries: direct cloning of DNA fragments generated by PCR, D. Kovalic and B. Weisblum; subtractive cDNA cloning using oligo(dT)30 - latex and PCR, E. Hara et al; expression cloning - PCR versus episomal vectors for rescue of transfected genes, K.S. Miller and M. Brudnak; use of PCR for constructing jumping libraries, R.P. Kandpal and S.M. Weissman. Part 8 Site-directed mutagenesis by PCR: site directed mutagenesis by PCR - substitution, insertion, deletion, and gene fusion, S. Barik; use of PCR in analysis of 5' - flanking region of androgen receptor gene, M.V. Kumar and D.J. Tindall. Part 9 Application of PCR in AIDS research: detection of HIV-1 in brain tissue of individuals with AIDS by in situ gene amplification, O. Bagasra and R.J. Pomerantz; estimation of genetic heterogeneity in primate T-cell lymphoma/leukemia viruses by PCR, D.K. Dube et al; use of PCR in detection of antisense transcripts in HTLV-I-infected patients and human T-cell lines, S. Dube et al. Part 10 Miscellaneous uses of PCR: direct chemiluminescent sequencing of double-stranded PCR products, A.M. Douglas and B.A. Atchison; use of PCR to determine genomic DNA target sites for zinc finger protein expressed in mouse cerebellum, A. Christoph and H.-J. Thiesen; DNA extraction from archived specimens by sonication, R.A. Robinson and M.J. Heller.