Part 1 Direct PCR on clinical specimens: formamide low-temperature PCR - applications for direct PCR from clinical material, M. Panaccio et al; direct use of blood in PCR, B.-Y. Nordvag et al. Part 2 Application of reverse transcription-mediated PCR: use of PCR for isolation of neuropeptide receptor genes, T.K. Chatterjee et al; lock-docking RACE PCR - strategies and applications, N.D. Borson et al; single-cell cDNA-PCR, B.L. Ziegler et al. Part 3 Gene synthesis by PCR: PCR-mediated synthesis of chimeric molecules, A. Darveau et al; PCR-mediated gene synthesis, K. Jayaraman. Part 4 Quantitative PCR: quantitative PCR - analysis of rare mitochondrial DNA mutations in central nervous system tissues, N.W. Soong and N. Arnheim; PCR-aided transcript titration assay - competitive PCR for evaluation of absolute levels of rare mRNA species, M. Becker-Andre; comparative evaluation of quantitative PCR methods, S. Sur et al. Part 5 Application of PCR in mutation detection: mutational detection by single-strand conformational polymorphism, N. Bardeesy and J. Pelletier; detection of mutation in yeast hsp60 gene by PCR, A. Sanyal and G.S. Getz; comparison of the sensitivity of single-strand conformational polymorphism and heteroduplex methods, D. Glavac and M. Dean; analysis of p53 mutations in human gliomas by RNA single-strand conformational polymorphism, T.M. Cheng et al. Part 6 Generation of probes by PCR: PCR and generation of antisense RNA probes for use in RNase protection assays, H. Yang and P.W. Melera; in vitro transcription of cRNA from PCR-generated DNA fragments, S. Leonard. Part 7 PCR in the context of cloning and constructing libraries: direct cloning of DNA fragments generated by PCR, D. Kovalic and B. Weisblum; subtractive cDNA cloning using oligo(dT)30 - latex and PCR, E. Hara et al; expression cloning - PCR versus episomal vectors for rescue of transfected genes, K.S. Miller and M. Brudnak; use of PCR for constructing jumping libraries, R.P. Kandpal and S.M. Weissman. Part 8 Site-directed mutagenesis by PCR: site directed mutagenesis by PCR - substitution, insertion, deletion, and gene fusion, S. Barik; use of PCR in analysis of 5' - flanking region of androgen receptor gene, M.V. Kumar and D.J. Tindall. Part 9 Application of PCR in AIDS research: detection of HIV-1 in brain tissue of individuals with AIDS by in situ gene amplification, O. Bagasra and R.J. Pomerantz; estimation of genetic heterogeneity in primate T-cell lymphoma/leukemia viruses by PCR, D.K. Dube et al; use of PCR in detection of antisense transcripts in HTLV-I-infected patients and human T-cell lines, S. Dube et al. Part 10 Miscellaneous uses of PCR: direct chemiluminescent sequencing of double-stranded PCR products, A.M. Douglas and B.A. Atchison; use of PCR to determine genomic DNA target sites for zinc finger protein expressed in mouse cerebellum, A. Christoph and H.-J. Thiesen; DNA extraction from archived specimens by sonication, R.A. Robinson and M.J. Heller.