Methods in Neurosciences: Gene Expression in Neural Tissues v. 9

Methods in Neurosciences: Gene Expression in Neural Tissues v. 9

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The study of gene expression in neural tissue brings to bear some of the newest and most productive scientific technology to one of the most exciting and fastest growing areas. This volume describes methods for the study of the expression of specific genes frequently found in very low abundance. The key features and benefits of this volume include: convenient benchtop format; methods presented for easy adaptation to new systems; comprehensive protocols included for in situ hybridization, comparison of in situ and immuno-chemical methods for the study of regulatory molecules; gene transfer; the study of the regulation of gene expression; the enhancer trap method; quantifying rare mRNAs; isolation and use of cell lines; the homology approach for receptor cloning; trouble shooting.
Specific protocols included for the assessment of the expression of the following genes: calbindin, corticotrop in-releasing hormone, cytokines and neurotrophic factors, dystropin, FMRF amide, glucose transporter, insulin-like growth factor interphotoreceptor retinoid binding protein, muscarinic acetylcholine receptor, neuropeptide Y, neurotrophin receptor, nerve growth factor and its receptor, and proopiomelanocort in (POMC), and weaver.
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Product details

  • Hardback | 479 pages
  • 182.88 x 238.76 x 27.94mm | 1,088.62g
  • Academic Press Inc
  • San Diego, United States
  • English
  • references, index
  • 0121852679
  • 9780121852672

Table of contents

In situ hybridization and immunohistochemical methods in study of regulatory molecules, M. Schalling; using mRNA in situ hybridization to localize Wnt-3 and Wnt-3A expression in developing neural tube, H. Roelink and r. Nusse; in situ hybridization for neurofilament and GAP-43 mRNA in primary sensory neurons in conjunction with nerve growth factor receptor radioautography, W. Tetzlaff, et al; gene transfer - gene regulation analysis by lipopolyamine-mediated DNA transfer in primary neurons, R. Barthel, et al; retrovirus-mediated gene transfer into mouse cerebellar cultures, M. Tsuda; analysis of Cis-acting elements of oxytocin gene by DNA-mediated gene transfer, S. Richard and H.H. Zingg; identification of Cis-regulatory sequences required for induction of neurotensin/neuromedin N gene expression in PC12 cells, E. Kislauskis and P.R. Dobner; characterization of neuron-specific transcription factors in drosophila melanogaster, W.A. Johnson; DNA - protein interactions in phenotypically identified neurons in discrete regions of intact brain, C.L. Szymeczek-Seay and D.E. Millhorn; general approaches - enhancer trap method in drosophila - its application to neurobiology, M. Mlodzik and Y. Hiromi; use fo Northern blotting for quanitifying rare mRNAs in neurons of developing peripheral nervous system, S. Wyatt and A.M. Davies; dopamine D2 receptors - isolation of cell lines using 2ligand binding, R.D. Todd, et al; cloning of dopamine receptors - homology approach, J.R. Bunzow, et al; cloning of human thyrotropin-releasing hormone cDNA using degenerate oligonucleotide probes and tetramethylammonium chloride, M.A. Tavianini, et al; in vivo analysis of gene expression in central catecholamine cells, V. Leviel and N.F. Biguet.
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