Methods in Molecular Genetics: v. 8
This volume is a practical guide to the application of molecular biology and genetic techniques to human cells. It includes detailed experimental protocols for: mutation detection in human genes - chemical mismatch clevage, DNA mini-sequencing, SSCP method, RT-PCR, electrophoretic mobility shift assay (EMSA), protein truncation test, chromosome deletion analysis; gene mapping, cloning and sequencing - gene linkage determination, large-capacity cloning system, cDNA isolation, differential display method, primer-baed DNA sequencing; transcription - promotors, transcription factors; RNA editing, ribozymes, antisense RNA - mammalian RNA editing assays, ribozymes as genetic tools, antisense RNA technology; genome recombination, amplification - recombination assays for mammalian cells, gene amplification measurement; receptors, signal transduction - intra-cellular receptor characterization, analysis of signal transduction genes; and the mouse as a model system for human molecular genetics - mouse genome methods (mouse crosses, somatic cell hybrids, YACs), mouse model for cardiovascular disease.
- Hardback | 487 pages
- 195 x 241 x 25.4mm | 1,121g
- 23 May 1996
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Table of contents
Mutation detection in human genes; gene mapping, cloning, sequencing; transcription - promoters, transcription factors, mRNA; RNA editing, ribozymes, antisense RNA; genome recombination, amplification; receptors, signal transduction; the mouse as a model system for human molecular genetics.