Methods in Molecular Genetics: Microbial Gene Techniques v. 6

Methods in Molecular Genetics: Microbial Gene Techniques v. 6

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A practical laboratory guide to current techniques of molecular biology and genetics. The focus of the volume is on microbial cells, particularly eukaryotic microbes and bacteria, as well as plasmids and bacteriophages.
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Product details

  • Hardback | 474 pages
  • 196.85 x 230 x 25.4mm | 1,107g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0120443082
  • 9780120443086

Table of contents

Part 1 Molecular genetics of eukaryotic microbes: forward and reverse genetics in the study of the obligate, intracellular parasite "toxoplasma gondii", J.C. Boothroyd et al; techniques associated with protozoan parasite genome analysis, J.W. Ajioka et al; "aspergillus nidulans" as a model system for cell and molecular biology studies, G.H. Goldman and N.R. Morris; special molecular techniques for study of filamentous fungi, G.A. Marzluf. Part 2 Yeast chromosomes - transcription, recombination, replication: "in vitro" fragmentation of yeast chromosomes and YACs at artificially inserted sites and applications to genome mapping, H. Tettelin et al; basic analysis of transcription factor binding to nucleosomes, J. Cote et al; experimental analysis of transcription factor - nucleosome interactions, M. Vettese-Dadey et al; mapping of yeast nucleosome "in vivo", M. Buttinelli et al; novel assays for ligation and cleavage activities of site-specific recombinases, P.D. Sadowski et al; pheromone procedures in fission yeast, J. Davey et al. Part 3 Bacterial gene structure and regulation: use of DNA methylation deficient strains in molecular genetics, L.J. Rasmussen and M.G. Marinus; identifying the ends of infrequent RNA molecules in bacteria, V.J. Cannistraro and D. Kennell; identification of "rcs" genes in "escherichia coli" 09:K30:H12 and their involvement in regulation of expression of the group IAK30 capsular polysaccharide, C. Whitfield et al; cloning of "thermomonospora fusca" cellulase genes in "escherichia coli" and "streptomyces lividans", D.B. Wilson; computational analyses aiding identification and characterization of proteins, genes and operons, M.H. Saier, Jr. and J. Reizer. Part 4 Plasmids and phages - replication, transcription, assembly: techniques to isolate specialized templates for "in vitro" transcription assays, S. Garges et al; use of coliphage lambda and other bacteriophages for molecular genetics analysis of "erwinia" and related gram-negative bacteria, V. Mulholland and G.P.C. Salmond. (Part contents).
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