Methods in Molecular Genetics: Gene and Chromosome Analysis v.5
This volume is a practical laboratory guide to current techniques of molecular biology and genetics. The focus in on eukaryotic cells, particularly human cells and other eukaryotic model systems. Detailed experimental protocols are provided for gene analysis, transcription analysis, transcription factors, splicing, differential display method, PCR, recombinant proteins, antibody methods, receptors, gene amplification strategies, chromosome analysis, yeast artificial chromosomes (YACs), microcloned DNA markers, somatic cell genetic methods, in situ hybridization, chromosomal proteins and DNA, chromatin reconstitution, telomerase and telomeres, nucleoproteins, DNA damage, disease and development, carcinogen analysis, plasimid DNA transfer, and mouse models.
- Hardback | 425 pages
- 152.4 x 228.6 x 25.4mm | 628.22g
- 01 Nov 1994
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Table of contents
Part 1 Gene analysis: method of differential display; analysis of active genes; transcriptional regulation of gene expression and characterization of gene promoters and transcription factors; in vivo protein-DNA interactions associated with gene transcription; analysis of T-Cell receptor transcripts by the nonpalindromic adaptor polymerase chain reaction; preparation and use of nuclear extracts from Drosophila cells for in vitro splicing. Part 2 Recombinant proteins: antibody-plasminogen activator conjugates and recombinant proteins; baculovirus-mediated overexpression of the glucocorticoid and mineralocorticoid receptors and related proteins; gene amplification strategies for protein production in mammalian cells. Part 3 Chromosome analysis: manipulation of large genomic constructs using yeast artificial chromosomes; the analysis of disease-specific chromosomal rearrangements with microcloned DNA markers; somatic cell genetic methods in human chromosome mapping; nonisotopic in situ hybridization in paraffin-embedded tissue sections. Part 4 Chromosomal proteins and DNA: biochemical reconstitution of chromatin with physiological nucleosome spacing; analysis of telomerase and telomeres; isolation, purification, and analysis of specific gene-containing nucleoproteins and nucleoprotein complexes; detection and identification of proteins which recognize DNA damage. Part 5 Disease and development: structural and functional analysis of tumour suppressor p53; SOS function tests for studies of chemical carcinogenesis using Salmonella typhimurium; analysis of plasmid DNA after transfer into mammalian tissues; application of mouse crosses toward defining the genetics of disease phenotypes; multiple strategies for isolating mouse homologues of developmental control genes of Drosophila.