Methods in Molecular Genetics: Gene and Chromosome Analysis v.1

Methods in Molecular Genetics: Gene and Chromosome Analysis v.1

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The new series, "Methods in Molecular Genetics", should provide experimental procedures for use in the laboratory. Because the introduction of molecular genetic techniques and related methodology has revolutionized biological research, a wide range of methods will be covered. The power and applicability of these techniques have led to detailed molecular answers to important biological questions and have changed the emphasis of biological research, as well as medical research, from the isolation and characterization of cellular material to studies of genes and their protein products. "Methods in Molecular Genetics" should be of value to researchers as well as students and technicians in a number of disciplines because of the wide applicability of the procedures and the topics that will be covered. The focus of this volume is on eukaryotic cells and, in particular, on human and other mammalian cells.
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Product details

  • Hardback | 435 pages
  • 196.85 x 230 x 25.4mm | 971g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0120443015
  • 9780120443017

Table of contents

Part 1 Gene Cloning: T Miki and SA. Aaronson, cDNA Cloning Method Using Stable Expression in Mammalian Cells. S. Parimoo, S.R. Patanjaii, and S.M. Weissman, Normalization and Selection with Short Fragment cDNAs. A.M. Lawler, C.H. Mjaatvedt, and J.D. Gearhart, Identification of Differentially Expressed Genes during Mammalian Embryogenesis: Subtraction Hybridization of Embryonic cDNA Libraries. P.G. Reddy, Z-Z Su, and P-B. Fisher, Identification and Cloning of Genes Involved in Progression of the Transformed Phenotype. W.C. Summers and P.M. Glazer, Lambda Phage-Based Systems for Rescue of Chromsomal Markers. Part 2 Cell Hybrids: A.A. Langston and R.E.K. Fournier, Preparation and Properties of Microcell Hybrids. K.A. Henning and E.C. Friedberg, Techniques and Applications of Microcell-Mediated Chromosome Transfer to Mammalian Cells. S.F. Dowdy and E.J. Stanbridge, Functional Analysis of Tumour Suppressor Genes.
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