Methods in Microbiology: Yeast Gene Analysis v. 26

Methods in Microbiology: Yeast Gene Analysis v. 26

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Yeast Gene Analysis, edited by two leading experts in the field, presents the most appropriate, up-to-date experimental approaches in detail. The layout is structured for ease of reference, and the volume will be essential reading for all researchers working in genomics, molecular biology, genetics, and microbiology. It provides a carefully selected collection of cutting-edge chapters reviewing alternative experimental approaches for the biologist wishing to study the function of genes identified by genome sequencing. A multi-authored book, it brings together experts from various areas of cell and molecular biology who provide insight into the analysis of genes in yeast. Features: * OF VOLUME: * Focuses on the methods most useful for the functional analysis of yeast genes * Allows researcher to identify which strategy to use without having to wade through numerous recipes * Includes techniques for mutagenesis, transcript analysis, transposon tagging, and use of reporter genes * Describes the use of tools for studying post-translational modifications in yeast * Includes useful appendices with handy basic yeast recipes and WWW addresses
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Product details

  • Hardback | 544 pages
  • 17 x 25 x 25.4mm | 980g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 69 illustrations, 1 colour figure
  • 0125215266
  • 9780125215268

Table of contents

Introduction to functional analysis of the yeast genome, S.G. Oliver; Whole chromosome analysis, E.J. Louis; The bioinformatics of the yeast genome, H.W. Mewes et al; Transformation of yeast by the lithium acetate/single-stranded carrier DNA/PEG method, R.D. Gietz, R.A. Woods; PCR-based gene targeting in saccharomyces cerevisiae, A. Wach et al; Studying essential genes-generating and using promoter fusions and conditional alleles, M.J.R. Stark; Ty mutagenesis, D.J. Garfinkel et al; Transcript analysis, A.J.P. Brown et al; Reporter genes and their use in studying yeast gene expression, N. Koloteva et al; Transposon tagging I - a novel system for monitoring protein production, function and localization, P. Ross-Macdonald et al; Transposon tagging II - exploration of gene function and regulatory networks in yeast with the mini-mu transposon, M. Bolotin-Fukuhara; Immunological approaches to the study of protein localisation in yeast, I.M. Hagan, C.J. Stirling; Post-translational modifications of secretory proteins, F.M. Klis et al; Studying post-translational modifications in yeast-intracellular proteins, M.J.R. Stark; Analysis of protein-protein interactions in yeast, J. Rosamond; 'Smart' genetic screens, J. de la Cruz et al; Metabolic control analysis as a tool in the elucidation of the function of novel genes, B. Teusink et al; Identifying stress genes, W.H. Mager et al; Identifying genes encoding components of the protein synthesis machinery of the yeast saccharomyces cerevisiae, M.F. Tuite et al; MAP kinase-mediated signal transduction pathways, M. Molina et al; The analysis of the candida albicans genome, P.T. Magee; Applications of yeast in the discovery of human gene function, P.A. Moore; Yeast mutant and plasmid collections, P. Kotter; Yeast gene analysis in the 21st century, A. Brown, M.F. Tuite. Appendices: The ribosomal protein genes of saccharomyces cerevisiae, R.J. Planta et al; Yeast growth, A.J.P. Brown, M.F. Tuite; Useful World Wide Web addresses for yeast researchers, A.J.P. Brown; nomenclature for yeast genes and proteins, M.F. Tuite.
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