Methods in Cell Biology: Xenopus Laevis: Practical Uses in Cell and Molecular Biology v.36

Methods in Cell Biology: Xenopus Laevis: Practical Uses in Cell and Molecular Biology v.36

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Description

A book intended for researchers, instructors, and students in cell biology, developmental biology, zoology, neurobiology, and molecular biology. This volume presents the first comprehensive treatment of the wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology. Each chapter includes background information, experimental protocols, and suggested applications. An extensive array of techniques is featured. The authors are experienced researchers who have written chapters intended to be useful to both other experienced researchers and to those new to Xenopus as an experimental system.
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Product details

  • Hardback | 646 pages
  • 160.02 x 228.6 x 40.64mm | 1,179.33g
  • Academic Press Inc
  • San Diego, United States
  • English
  • colour plates and diagrams, references, index
  • 0125641362
  • 9780125641364

Table of contents

Raising xenopus in the laboratory, M. Wu and J. Gerhart; genetics of xenopus laevis, J.D. Graf and H.R. Kobel; experimentally induced homozygosity in xenopus laevis, R. Tompkins and D. Reinschmidt; oogenesis and oocyte isolation, L.D. Smith; early embryonic development of xenopus laevis, R. Keller. Part 2 Oocytes - vitellogenin uptake in vitro culture of oocytes, L.K. Opresko; biochemical fractionation of oocytes, J.P. Evans and B.K. Kay; lampbrush chromosomes, J.G. Gall et al; preparation of synthetic mRNAs and analyses of translational efficiency of microinjected xenopus oocytes, M. Wormington; the use of oligonucleotides for antisense experiments in xenopus laevis oocytes, C. Prives and D. Foukal. Part 3 Embryos: fertilization of cultured xenopus oocytes and use is studies of maternally inherited molecules, J. Heasman et al; isolation of extracellular matrix structures from xenopus laevis oocytes, eggs, and embryos, J.L. Hedrick and D.M. Hardy; analysis of cellular signalling events, the cytoskeleton, and spatial organizations of macromolecules during early xenopus development, D.G. Capco and W.M. Bement; generation of body plan phenotypes in early embryogenesis, K. Kao and M. Danilcluk; fluorescent dextran clonal markers, R.L. Gimlich; nuclear transplantation in xenopus, J.B. Gurdon; mesoderm induction, I.B. Dawid; neural induction, C.R. Phillips; analysis of class II gene regulation, T.D. Sargent and P.H. Mathers; assays for gene function in developing xenopus embryos, P.D. Vize et al; histological preparation of xenopus laevis oocytes and embryos, C.M. Kelly et al; whole-mount staining of xenopus and other vertebrates, M.W. Klymkowsky and J. Hanker; in situ hyridization, H.P. O'Keefe (part contents).
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