Methods in Cell Biology: Protein Expression in Animal Cells v. 43
Protein Expression in Animal Cells presents information essential to researchers and students investigating cellular function. This exceptional laboratory guide covers the selection and use of expression systems that are optimal for specific exogenous proteins. Current studies often require protein expression in particular cell types, in amounts suitable for specific detection methods, and under conditions that do not otherwise perturb the specific cellular mechanisms under investigation. Such demanding requirements imply tht there is no universal vector for all experiments. This comprehensive manual describes the structure, production, host range, and practical use of diverse viral and plasmid vectors. Protocols are written at a level geared to the beginning lab student. Because it covers many versatile expression systems, expert researchers will also find this guidebook a valuable addition to their laboratory.
- Spiral bound | 366 pages
- 198.1 x 233.7 x 17.8mm | 839.16g
- 10 Nov 1994
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Table of contents
Part 1 The use of naturally occurring and recombinant viruses: viruses as model systems in cell biology, R.W. Compans and P.C. Roberts; expression of exogenous proteins in mammalian cells with the recombinant simliki forest virus vector, V.M. Olkkonen et al; recombinant sindbis virus as an expression system for cell biology, R.C. Piper et al; recombinant rous sarcoma virus vectors for avian cells, G. Odorizzi and I.S. Trowbridge; generation of high-titer pseudotyped retroviral vectors with very broad host range, J.-K. Lee et al; SV40 virus expression vectors, H.Y. Naim and M.G. Roth; use of recombinant vaccinia virus vectors for cell biology, O.A. Weisz and C.E. Machamer; use of recombinant adenovirus for metabolic engineering of mammalian cells, T.C. Becker et al; amplicon-based herpes simplex virus vectors, D.Y. Ho; replication-defective recombinant herpes virus vectors, P.A. Johnson and T. Friedman. Part 2 The use of expression plasmids in continuous cell lines: CMV plasmid vectors for permanent lines of polarized epithelial cells, C.B. Brewer; inducible protein expression using a glucocorticoid-sensitive vector, R.P. Hirt et al; expression of exogenous proteins in cells with regulated secretory pathways, R.A. Chavez et al; expression of foreign proteins in a human neuronal system, D.G. Cook et al; homologous recombination for gene replacement in mouse cell lines, T.E. Willnow et al; regulation of protein activities by fusion to steroid binding domains, T. Mattioni et al; gene gun transfection of animal cells and genetic immunization, D.-C. Tang and S.A. Johnston; amplification of expression plasmids in continuous CHO cell lines and analysis of transdominant mutant proteins, I. Mellman.