Methods in Cell Biology: Methods in Avian Embryology v.51
This volume presents a combination of classical embryological techniques and modern molecular biological approaches to studying the developing avian embryo. It also describes how to use these methods to study problems in cell, developmental and neurobiology. The protocols emphasize microsurgery, histology, and cellular and molecular marking. The methods include: embryonic transplantations, cell culture and organ culture, in situ hybridization, classical histological techniques, and retrovirally mediated gene transfer.
- Spiral bound | 354 pages
- 205.74 x 238.76 x 25.4mm | 793.78g
- 01 May 1996
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Table of contents
Culture and Microsurgical Manipulation of the Early Avian Embryo. Quail-Chick Transplantations. Manipulations of Neural Crest Cells of Their Migratory Pathways. Manipulation of the Avian Segmental Plat in Vivo. Somite Strips: An Embryo Fillet Preparation. Embryo Slices. Operations on Limb Buds of Avian Embryos. Cell Marking and Image Analysis. Gene Transfer Using Replication-Defective Retrovital and Adenoviral Vectors. Manipulating Gene Expression with Replication Competent Retroviruses. Hybridization Analysis of Chick Embryos in Whole Mount and Tissue Sections. Micromass Cultures of Limb and Other Mesenchyme. Organ Cultures and Chorioallantoic Grafts. Autonomic and Sensory Neuron Cultures. Retinal Cultures. Migration and Adhesion Assays. Classical Histology and Cell Birthdating. Time Lapse Cinematography.