Methods in Cell Biology: Functional Organization of the Nucleus - A Laboratory Guide v. 35

Methods in Cell Biology: Functional Organization of the Nucleus - A Laboratory Guide v. 35

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A text intended for researchers and students in cell biology, genetics and molecular biology, developmental biology, biochemistry and biophysics. The complex problem of the relationship between nuclear structure and function demands a multidisciplinary, multifaceted approach. This laboratory guide is designed for researchers, from graduate students to professors, who need detailed protocols and general discussions on a broad range of techniques. The volume presents a selection of different methodological approaches for the analysis of nuclear structure and function - from cytological to molecular to genetic. These include visualization of nucleic acid sequences using hybridization probes, visualization of proteins using immunological probes, isolation of chromatin fractions, mapping "in vitro" protein-DNA interactions, reconstitution of functional templates and nuclear substructures, and genetic approaches to identifying and characterizing chromosomal components.
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Product details

  • Hardback | 590 pages
  • 152.4 x 228.6 x 25.4mm | 628.22g
  • Academic Press Inc
  • San Diego, United States
  • English
  • references, index
  • 0125641354
  • 9780125641357

Table of contents

DNA sequence localization in metaphase and interphase cells by fluorescence "in situ" hybridization, B.J. Trask; localization of mRNAs by "in situ" hybridization, L.M. Angerer and R.C. Angerer; fluorescent detection of nuclear RNA and DNA - implications for genome organization, C.V. Johnson et al; visualization of DNA sequences in meiotic chromosomes, P.B. Moens and R.E. Pearlman; nucleic acid sequence localization by EM "in situ" hybridization, S. Narayanswami et al; the use of autoantibodies in the study of nuclear and chromosomal organization, W. C. Earnshaw and J.B. Rattner; meiotic chromosome preparation and protein labeling, C. Heyting and A.J.J. Dietrich; distribution of chromosomal proteins in polytene chromosomes of "drosophila", R.F. Clark et al; the use of monoclonal antibody libraries, H. Saumweber; optical sectioning and three-dimensional reconstruction of diploid and polytene nuclei, M.C. Rykowski; yeast minichromosomes, S.Y. Roth and R.T. Simpson; nucleosomes of transcriptionally active chromatin - isolation of template-active nucleosomes by affinity chromatography, V.G. Allfrey and T.A. Chen; the nucleoprotein hybridization method for isolating active and inactive genes as chromatin, C. Vincenz et al; protein-DNA crosslinking as a means to determine the distribution of proteins on DNA "in vivo", D.S. Gilmour et al; protein-DNA interactions "in vivo" - examining genes in "saccharomyces cerevisiae" and "drosophila melanogaster" by chromatin footprinting, M.W. Hull et al; control of class II gene transcription during "in vitro" nucleosome assembly, J.L. Workman et al; systems for the study of nuclear assembly, DNA replication and nuclear breakdown in "xenopus laevis" egg extracts, C. Smythe and J.W. Newport; "in vitro" nuclear protein import using permeabilized mammalian cells, S.A. Adam et al; mutations that affect chromosomal proteins in yeast, M.M. Smith; mutations that affect nuclear organization in yeast, A.O. Sperry et al; mutations that affect cell division in "drosophila", M. Gatti and M.L. Goldberg; position effect variegation - an assay for mutations in chromosomal proteins and chromatin assembly in "drosophila", T. Grigliatti.
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