Methods in Cell Biology: Functional Organization of the Nucleus - A Laboratory Guide v. 35
The complex problem of the relationship between nuclear structure and function demands a multidisciplinary, multifaceted approach. This laboratory guide is designed for researchers, from graduate students to professors, who need detailed protocols and general discussions on a broad range of techniques. The volume presents a selection of different methodological approaches for the analysis of nuclear structure and function - from cytological to molecular to genetic. These include visualization of nucleic acid sequences using hybridization probes, visualization of proteins using immunological probes, isolation of chromatin fractions, mapping in vitro protein-DNA interactions, reconstitution of functional templates and nuclear substructures, and genetic approaches to identifying and characterizing chromosomal components.
- Paperback | 590 pages
- 164 x 231mm | 926g
- 01 Jan 1992
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Table of contents
DNA sequence localization in metaphase and interphase cells by fluorescence in situ hybridization, B. J. Trask; localization of mRNAs by in situ hybridization, L. M. Angerer and R. C. Angerer; fluorescent detection of nuclear RNA and DNA - implications for genome organization, C. V. Johnson et al; visualization of DNA sequences in meiotic chromosomes, P. B. Moens and R. E. Pearlman; nucleic acid sequence localization by EM in situ hybridization , S. Narayanswami et al; the use of autoantibodies in the study of nuclear and chromosomal organization, W. C. Earnshaw and J. B. Rattner; meiotic chromosome preparation and protein labeling, C. Heyting and A. J. J. Dietrich; distribution of chromosomal proteins in polytene chromosomes of drosphila, R. F. Clark et al; the use of nonoclonal antibody libraries, H. Saumweber; optical sectioning and three-dimensional reconstruction of diploid and polytene nuclei, M. C. Rykowski; yeast minichromosomes, S. Y. Roth and R. T. Simpson; nucleosomes of transcriptionally active chromatic - isolation of template-active nucleosomes by affinity chromatography, V. G. Alfrey and T. A. Chen; the nucleoprotein hybridization method for isolating active and inactive genes as chromatin, C. Vincenz et al; protein-DNA crosslinking as a means to determine the distribution of proteins on DNA in vivo, D. S. Gilmour et al; protein-DNA interactions in vivo - examining genes in saccharaomyces cerevisiae and drosophila melanogaster by chromatin footprinting, M. W. Hull et al; control of Class II gene transcription during in vitro nucleosome assembly, J. L. Workman et al; systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in xenopus laevis egg extracts, C. Smythe and J. W. Newport; in vitro nuclear protein import using permeabilized mammalian cells, S. A. Adam et al; mutations that affect chromosomal proteins in yeast, M. M. Smith; mutations that affect nuclear organization in yeast, A. O. Sperry et al; mutations that affect cell division in drosophila, M. Gatti and M. L. Goldberg; position effect variegation - an assay for mutations in chromosomal proteins and chromatin assembly in drosophila, T. Grigliatti.