Methods in Cell Biology: Flow Cytometry v.42

Methods in Cell Biology: Flow Cytometry v.42

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This is the second of a two volume set that provides a laboratory guide and reference on methods in flow cytometry sample preparation and analysis. The theoretical foundations, critical aspects and possible pitfalls of each procedure is outlined, and protocols are supplemented with explanatory sections and trouble-shooting tips. Various cell systems are described and ten methods cover assessment of apoptosis and other modes of cell death. The methods detailed should be accessible to researchers and students in biomedical science and biology who use flow cytometry to separate and analyze cells.
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Product details

  • Hardback | 627 pages
  • 199 x 235mm | 1,497g
  • Academic Press Inc
  • San Diego, United States
  • Revised
  • 2nd Revised edition
  • index
  • 0125641435
  • 9780125641432

Table of contents

A flow cytometric technique for detection of DNA repair in mammalian cells, J.R. Selden and F. Dolbeare; rapid determination of cellular resistance related drug efflux in tumour cells, A. Krishan; glutathione and cellular resistance to anti-cancer drugs, D. Hedley and S. Chow; assay of cell resistance to Ara-C, F. Lacombe; detection of mRNA species by flow cytometry, F. Belloc; PRimed IN Situ labelling (PRINS) and Fluorescent In Situ Hybridization (FISH), H. Fischer et al; molecular phenotyping by flow cytometry, B.D. Li et al; isolation and analysis of somatic cell mutants with defects in endocytic traffic, S.A. Brockman and R.F. Murphy; measurement of micronuclei by flow cytometry, M. Nusse et al; sperm chromatin structure assay (DNA denaturability), D. Evenson and L. Jost; fine-needle cytopuncture and flow cytometry in the characterization of human tumours, F. Spyratos and M. Briffod; functional NK assay using flow cytometry, S. Papa and M. Valentini; antibodies to intermediate filament proteins as probes for multi-parameter flow cytometry of human cancers, T.V. Shankey; interactive data analysis for evaluation of B-cell neoplasia by flow cytometry, R.E. Duque; analysis and sorting of hematopoietic stem cells from mouse bone marrow, J.M.W. Visser and P. de Vries; reticulocyte analysis and reticulocyte maturity index, B.H. Davis and N.C. Bigelow; analysis of platelets by flow cytometry, K.A. Ault and J. Mitchell; flow cytometry in malaria detection, C.J. Janse and P.H. Van Vianen; large-scale chromosomes sorting, J.J. Fawcett et al; strategies for rare cell detection and isolation, J.F Leary; cell sorting of biohazardous specimens for assay of immune function, J.V. Giorgi; high-speed photodamage cell sorting - an evaluation of the ZAPPER prototype, J.F. Keij et al; high-gradient magnetic cell sorting, A. Radbruch et al; contributions of flow cytometry to studies with multicell spheroids, R.E. Durand; functional measurements using HL-60 cells, J.P. Robinson et al; HIV infection - diagnosis and disease progression evaluation, J.V. Giorgi and A. Landay; cell cycle analysis of "saccharomyces cerevisiae", B.S. Dien et al; staining and measurement of DNA in bacteria, H.B. Steen et al; detection of specific microorganisms, G. Vesey et al; strategies for flow cytometric analysis of marine microalgae and sponge cells, C.M. Yentsch and S.A. Pomponi; flow cytometry and sorting of plant protoplasts and cells, D.W. Galbraith; use of fluorescence-activated cell sorting for rapid isolation of insect cells harbouring recombinant baculovirus, M.A. Sommerfelt and E.J. Sorscher; flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes, T.M. McHugh; calibration of flow cytometer detector systems, R.E. Durand. (Part contents).
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