Methods in Cell Biology: Flow Cytometry v.42

Methods in Cell Biology: Flow Cytometry v.42

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Description

Flow Cytometry, Second Edition is a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow cytometry to separate and analyze cells.
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Product details

  • Paperback | 627 pages
  • 201.4 x 235.7 x 34mm | 1,662.13g
  • Academic Press Inc
  • San Diego, United States
  • English
  • Revised
  • 2nd Revised edition
  • index
  • 0122030524
  • 9780122030529

Table of contents

A flow cytometric technique for detection of DNA repair in mammalian cells, J.R. Selden and F. Dolbeare; rapid determination of cellular resistance related drug efflux in tumour cells, A. Krishan; glutathione and cellular resistance to anti-cancer drugs, D. Hedley and S. Chow; assay of cell resistance to Ara-C, F. Lacombe; detection of mRNA species by flow cytometry, F. Belloc; PRimed IN Situ labelling (PRINS) and Fluorescent In Situ Hybridization (FISH), H. Fischer et al; molecular phenotyping by flow cytometry, B.D. Li et al; isolation and analysis of somatic cell mutants with defects in endocytic traffic, S.A. Brockman and R.F. Murphy; measurement of micronuclei by flow cytometry, M. Nusse et al; sperm chromatin structure assay (DNA denaturability), D. Evenson and L. Jost; fine-needle cytopuncture and flow cytometry in the characterization of human tumours, F. Spyratos and M. Briffod; functional NK assay using flow cytometry, S. Papa and M. Valentini; antibodies to intermediate filament proteins as probes for multi-parameter flow cytometry of human cancers, T.V. Shankey; interactive data analysis for evaluation of B-cell neoplasia by flow cytometry, R.E. Duque; analysis and sorting of hematopoietic stem cells from mouse bone marrow, J.M.W. Visser and P. de Vries; reticulocyte analysis and reticulocyte maturity index, B.H. Davis and N.C. Bigelow; analysis of platelets by flow cytometry, K.A. Ault and J. Mitchell; flow cytometry in malaria detection, C.J. Janse and P.H. Van Vianen; large-scale chromosomes sorting, J.J. Fawcett et al; strategies for rare cell detection and isolation, J.F Leary; cell sorting of biohazardous specimens for assay of immune function, J.V. Giorgi; high-speed photodamage cell sorting - an evaluation of the ZAPPER prototype, J.F. Keij et al; high-gradient magnetic cell sorting, A. Radbruch et al; contributions of flow cytometry to studies with multicell spheroids, R.E. Durand; functional measurements using HL-60 cells, J.P. Robinson et al; HIV infection - diagnosis and disease progression evaluation, J.V. Giorgi and A. Landay; cell cycle analysis of "saccharomyces cerevisiae", B.S. Dien et al; staining and measurement of DNA in bacteria, H.B. Steen et al; detection of specific microorganisms, G. Vesey et al; strategies for flow cytometric analysis of marine microalgae and sponge cells, C.M. Yentsch and S.A. Pomponi; flow cytometry and sorting of plant protoplasts and cells, D.W. Galbraith; use of fluorescence-activated cell sorting for rapid isolation of insect cells harbouring recombinant baculovirus, M.A. Sommerfelt and E.J. Sorscher; flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes, T.M. McHugh; calibration of flow cytometer detector systems, R.E. Durand. (Part contents).
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Review quote

The second edition of Flow Cytometry provides a comprehensive and detailed foundation in the application of this diverse and rapidly expanding technology. The variety and scope of topics covered in this two-volume set are too large to cover in detail. The second edition of Flow Cytometry provides the investigator with a concise compilation of new and updated applications and procedures, updated references and a directory of the principal contributors to the field. The additions and improvements to the second edition are evidence of the impact this technology will continue to have on biological and medical sciences for years to come. --AMERICAN SCIENTIST The Second Edition of this valuable book, nearly twice the size of the first, testifies to the continuing extraordinary growth of new biologic applications for flow cytometry. This Methods book fills the need for a compendium of those techniques, presented in concise, straightforward language. It is a highly readable compendium of biologic techniques for flow cytometry that should be in every laboratory... --Myron R. Melamed in CYTOMETRY @from:Reviews of the First Edition: This is a good reference manual for a multi-user facility faced with a wide variety of biological applications. --CYTOMETRY Flow Cytometry includes an impressive array of methods applicable to chromosome analysis, plant biology, marine biology, fluorescence, in situ hybridization, and others. It succeeds in providing the reader with good insight into the power of the technology throughout biology. --KENNETH A. AULT, Maine Cytometry Research Institute, Maine Medical Center, in CANCER CELLS The editors, who are themselves major contributors, have assembled a prodigious list of 140 contributors. Virtually all who have developed or refined the techniques of flow cytometry over the last decade or more have been recruited to describe the optimum methodologies. It is a highly readable compendium of biological techniques for flow cytometry that should be in every laboratory, and priced at a bargain. --CANCER RESEARCH INSTITUTE Every chapter has a clear introduction to the background of the method under discussion comprehensible to the inexperienced reader. Applications are very complete... --CELL BIOLOGY INTERNATIONAL
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About Zbigniew Darzynkiewicz

John P. Robinson is a Professor of Sociology at the University of Maryland, College Park. Dr. Robinson founded and directed the Survey Research Center at the University of Maryland and the Communication Research Center at Cleveland State University. He has published more than 100 articles in professional journals and books and is a contributing editor to American Demographics magazine. Dr. Robinson was an American Statistical Association/National Science Foundation fellow at the Bureau of Labor Statistics in 1992-93, a Fulbright scholar at Moscow State University and Soviet Academy of Sciences in 1990, a Research Consultant at BBC News in 1978 and Research Coordinator for the U.S. Surgeon General's Program on Television and Human Behavior in 1970. He received the 1987 Fordham University McGannon Award for Social and Ethical Relevance in Communication Policy Research for his research on improving public understanding of the news. His areas of specialization include social science methodology, attitude and behavior measurement, social change, and the impact of mass communication and other home technology. Additionally, he is an Editor of the Encyclopedia of Food Science, Food Technology & Nutrition.
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