Methods in Cell Biology: Flow Cytometry v.41

Methods in Cell Biology: Flow Cytometry v.41

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Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.
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Product details

  • Spiral bound | 534 pages
  • 208 x 236.2 x 31.2mm | 1,423.26g
  • Academic Press Inc
  • San Diego, United States
  • English
  • Revised
  • 2nd Revised edition
  • index
  • 0122030516
  • 9780122030512

About Zbigniew Darzynkiewicz

John P. Robinson is a Professor of Sociology at the University of Maryland, College Park. Dr. Robinson founded and directed the Survey Research Center at the University of Maryland and the Communication Research Center at Cleveland State University. He has published more than 100 articles in professional journals and books and is a contributing editor to American Demographics magazine. Dr. Robinson was an American Statistical Association/National Science Foundation fellow at the Bureau of Labor Statistics in 1992-93, a Fulbright scholar at Moscow State University and Soviet Academy of Sciences in 1990, a Research Consultant at BBC News in 1978 and Research Coordinator for the U.S. Surgeon General's Program on Television and Human Behavior in 1970. He received the 1987 Fordham University McGannon Award for Social and Ethical Relevance in Communication Policy Research for his research on improving public understanding of the news. His areas of specialization include social science methodology, attitude and behavior measurement, social change, and the impact of mass communication and other home technology. Additionally, he is an Editor of the Encyclopedia of Food Science, Food Technology & Nutrition.
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Table of contents

Dissociation of intact cells from tumours and normal tissues, D.W. Visscher and J.D. Crissman; assays of cell viability - discrimination of cells dying by apoptosis, Z. Darzynkiewicz et al; cell preparation for the identification of leukocytes, C.C. Stewart; multiparameter analysis of leukocytes by flow cytometry, C.C. Stewart; immunophenotyping using fixed cells, G.F. Babcock and S.M. Dawes; simultaneous DNA content and cell surface immunofluorescence analysis, P.P.T. Brons et al; flow cytometry crossmatching for solid organ transplantation, R.A. Bray; cell membrane potential analysis, H.M. Shapiro; measurement of intracellular pH, M.J. Boyer and D.W. Hedley; intracellular ionized calcium, C.H. June and P.S. Rabinovitch; cellular protein content measurements, H.A. Crissman and J.A. Steinkamp; lysosomal proton pump activity - supravital cell staining with acridine orange differentiates leukocyte subpopulations, F. Traganos and Z. Darzynkiewicz; staining of DNA in live and fixed cells, H.A. Crissman and G.T. Hirons; high-resolution analysis of nuclear DNA employing the fluorochrome DAPI, F.J. Otto; detergent and proteolytic enzyme based techniques for nuclear isolation and DNA content analysis, L.L. Vindelov and I.J. Christensen; DNA analysis from paraffin-embedded blocks, D.W. Hedley; controls, standards and histogram interpretation in DNA flow cytometry, L.G. Dressler and L.C. Seamer; DNA content histogram and cell cycle analysis, P.S. Rabinovitch; immunochemical quantitation of bromodeoxyuridine - application to cell cycle kinetics, F. Dolbeare and J.R. Selden; application and detection of IdUrd and CldUrd as two independent cell cycle markers, J.A. Aten et al; cell cycle analysis using continuous bromodeoxyuridine labelling and Hoechst 33358-ethidium bromide bivariate flow cytometry, M. Poot et al; detection of BrdUrd-labelled cells by differential analysis of DNA fluorochromes - pulse-chase and continuous labelling methods, H.A. Crissman et al; analysis of intracellular proteins, K.D. Bauer and J.W. Jacobberger; "washless" procedures for nuclear antigen detection, J.K. Larsen; light scatter of isolated cell nuclei as a parameter discriminating the cell cycle subcompartments, W. Giaretti and M. Nusse; simultaneous analysis of cellular RNA and DNA content, Z. Darzynkiewicz; analysis of DNA content and cyclin proteins expression in studies of DNA ploidy, growth fraction, lymphocyte stimulation and cell cycle, Z. Darzynkiewicz et al; oxidative product formation analysis by flow cytometry, J.P. Robinson et al; flow cytometric determination of cysteine and serine proteinase activities in living cells with rhodamine 110 substrates, S. Klingel et al; leucine aminopeptidase activity by flow cytometry, J.J. Turek and J.P. Robinson; enzyme kinetics, J.V. Watson and C. Dive; on-line flow cytometry - a versatile method for kinetic measurements, K. Nooter et al. (Part contents).
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Review quote

This is a good reference manual for a multi-user facility faced with a wide variety of biological applications. --CYTOMETRY The second edition of Flow Cytometry provides a comprehensive and detailed foundation in the application of this diverse and rapidly expanding technology. The variety and scope of topics covered in this two-volume set are too large to cover in detail. The second edition of Flow Cytometry provides the investigator with a concise compilation of new and updated applications and procedures, updated references and a directory of the principal contributors to the field. The additions and improvements to the second edition are evidence of the impact this technology will continue to have on biological and medical sciences for years to come. --AMERICAN SCIENTIST Flow Cytometry includes an impressive array of methods applicable to chromosome analysis, plant biology, marine biology, fluorescence, in situ hybridization, and others. It succeeds in providing the reader with good insight into the power ofthe technology throughout biology. --KENNETH A. AULT, Maine Cytometry Research Institute, Maine Medical Center, in CANCER CELLS Every chapter has a clear introduction to the background of the method under discussion comprehensible to the inexperienced reader. Applications are very complete. --B. Lambrecht-Ghent in CELL BIOLOGY INTERNATIONAL
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