Methods in Cell Biology: Antibodies in Cell Biology v. 37
"Antibodies in Cell Biology" focuses on a new generation of protocols aimed at the cell biologist. This laboratory manual features systems and techniques that are especially relevant for modern problems. The contributing authors have been carefully chosen for their specific expertise, and have provided detailed protocols, recipes, and trouble-shooting guides in each chapter. The book is designed for any researcher or student who needs to use antibodies in cell biology and related research areas.
- Paperback | 420 pages
- 198.12 x 228.6 x 10.16mm | 793.78g
- 01 Sep 1993
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Table of contents
The immune response - an overview, E.S. Golub; production of polyclonal antibodies against proteins and peptides, D. Drenckhahn T. Jons and F. Schmitz; making monoclonal antibodies, D.J Asai and J.K. Wilder; purification of mouse antibodies and fab fragments, T.J. Smith; blot-affinity purification of antibodies, W.-J.Y. Tang; immunoblotting, J.J. Otto; immunoprecipitation methods, J.J. Otto and S-W. Lee; a quantitative solid-phase binding assay for tubulin, D. Thrower, et al; ultra small glod probes - characteristics and use in immuno(cyto)chemical studies, P. van de Plas and J.L.M. Leunissen; confocal immunofluorescence microscopy of microbubules in oocytes, eggs, and embryos of algae and amphibians, D.L. Gard and D.L. Kropf; immunofluorescence localization of antigens in plant cells, S.M. Wick; antigen localization in fission yeast, C.E. Alfa, et al; non-fluorescent immunolocalization of antigens in mitotic sea urchin bnlasotomeres, B.D. Wright and J.M. Scholey; immuno-gold electron microscopy - mapping tubulin isotypes on neurite microtubules, H.C. Joshi; post-embedding immunocytochemical techniques for light and electron microscopy, S.K. Fisher; EM immunocytochemistry following cryofixation and freeze substitution, J.Z. Kiss an K. McDonald; microinjection of antibodies, B.M. Jockusch.