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Mast Cells : Methods and Protocols

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A cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells. These methods take advantage of the latest advances in molecular biology, technology, and information science. They include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis.
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Product details

  • Hardback | 439 pages
  • 156 x 236 x 26mm | 879.98g
  • Totowa, NJ, United States
  • English
  • 2005 ed.
  • 115 Illustrations, black and white; XVIII, 439 p. 115 illus.
  • 1588293742
  • 9781588293749

Back cover copy

The mast cell, long implicated in causing allergic reactions, may also be involved in many other disease processes, including cancer, heart disease, parasitic disease, atherosclerosis, asthma, and arthritis. In Mast Cells: Methods and Protocols, hands-on experts describe in detail their best techniques for the isolation, culture, and study of both activation and signaling in human mast cells. These readily reproducible methods take advantage of the latest advances in molecular biology, technology, and information science. The techniques provide a sound base of methodology for mast cell research and include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis. A survey of mast cell biology offers insight into its history and the implications for adaptive immunity. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Mast Cells: Methods and Protocols provides mast cell researchers with reproducible accounts of basic and advanced molecular and cellular techniques used in studying this fascinating, multifunctional cell.
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Table of contents

Part I. Overview of Mast Cell Biology

Paul Ehrlich's 'Mastzellen'-From Aniline Dyes to DNA Chip Arrays: A Historical Review of Developments in Mast Cell Research
Harsha Vyas and Guha Krishnaswamy

The Human Mast Cell: An Overview
Guha Krishnaswamy, Omar Ajitawi, and David S. Chi

Mast Cells in Allergy and Autoimmunity: Implications for Adaptive Immunity
Gregory D. Gregory and Melissa A. Brown

Part II. Identification of Mast Cells in Culture and in Tissue

Analysis of MCT and MCTC Mast Cells in Tissue
Lawrence B. Schwartz

Mast Cell Ultrastructure and Staining in Tissue
Shruti A. Shukla, Ranjitha Veerappan, Judy S. Whittimore, Lou Ellen Miller, and George A. Youngberg

Expression of Cell Surface Antigens on Mast Cells: Mast Cell Phenotyping
Alexander W. Hauswirth, Stefan Florian, Gerit-Holger Schernthaner, Maria-Theresa Krauth, Karoline Sonneck, Wolfgang R. Sperr, and Peter Valent

Identification of Mast Cells in the Cellular Response to Myocardial Infarction
Nikolaos G. Frangogiannis and Mark L. Entman

Part III. Development of Mast Cells In Vitro

Growth of Human Mast Cells From Bone Marrow and Peripheral Blood-Derived CD34+ Pluripotent Progenitor Cells
Arnold S. Kirshenbaum and Dean D. Metcalfe

Culture of Human Mast Cells From Hemopoietic Progenitors
Hirohisa Saito

Isolation, Culture, and Characterization of Intestinal Mast Cells
Gernot Sellge and Stephan C. Bischoff

Part IV. Mast Cell Signaling and Gene Expression

Activation of Nuclear Factor-kB
Chuanfu Li, Jim Kelley, and Tuanzhu Ha

Analysis of Mitogen-Activated Protein Kinase Activation
Stephen C. Armstrong

Microarray and Gene-Clustering Analysis
Tsung-Hsien Tsai, Denise M. Milhorn, and Shau-Ku Huang

Techniques to Study FceRI Signaling
Yuko Kawakami, Jiro Kitaura, and Toshiaki Kawakami

Part V. Mast Cell Expression and Release of Inflammatory Mediators

Human Mast Cell Proteases: Activity Assays Using Thiobenzyl Ester Substrates
David A. Johnson

Mast Cell Histamine and Cytokine Assays
David S. Chi, S. Matthew Fitzgerald, and Guha Krishnaswamy

Measurement of Mast Cell Cytokine Release by Multiplex Assay
Kevin F. Breuel and W. Keith De Ponti

Assays for Histamine-Releasing Factors: From Identification and Cloning to Discovery of Binding Partners
Jacqueline M. Langdon and Susan M. MacDonald

Assays for Nitric Oxide Expression
William L. Stone, Hongsong Yang, and Min Qui

Immunohistological Detection of Growth Factors and Cytokines in Tissue Mast Cells
Zhenhong Qu

Part VI. Mast Cell Interactions With Other Cell Types

Endothelial Cell Activation by Mast Cell Mediators
Kottarappat N. Dileepan and Daniel J. Stechschulte

Co-Culture of Mast Cells With Fibroblasts: A Tool to Study Their Crosstalk
Ido Bachelet, Ariel Munitz, and Francesca Levi-Schaffer

Detection of e Class Switching and IgE Synthesis in Human B Cells
Jerome Pene, Florence Guilhot, Isabelle Cognet, Paul Guglielmi, Angelique Guay-Giroux, Jean-Yves Bonnefoy, Greg C. Elson, Hans Yssel, and Jean-Francois Gauchat

Part VII. Novel Aspects of Mast Cell Activation and Regulation

Gene Silencing Using Small Interference RNA in Mast Cells
Deling Yin and Charles A. Stuart

Mast Cell Activation by Lipoproteins
Jim Kelley, Gregory Hemontolor, Walid Younis, Chuanfu Li, Guha Krishnaswamy, and David S. Chi

Mast Cell Activation by Stress
Ann L. Baldwin

Part VIII. Roles of Mast Cells in Host Defense

In Vivo Models for Studying Mast Cell-Dependent Responses to Bacterial Infection
Christopher P. Shelburne, James B. McLachlan, and Soman N.
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Review Text

From the reviews:

"This volume provides a set of protocols for investigators working with mast cells. Each of the chapters is accompanied by a short bibliography. ... The information will be of value to advanced students and laboratory investigators. ... Each of the substantive chapters contains detailed protocols and a short set of primary references. ... there is sufficient detail to allow direct adaptation in the laboratory. ... the volume contains useful material for investigators working in this area." (Eugene A Davidson, Doody's Book Review Service, March, 2006)
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Review quote

From the reviews:





"This volume provides a set of protocols for investigators working with mast cells. Each of the chapters is accompanied by a short bibliography. ... The information will be of value to advanced students and laboratory investigators. ... Each of the substantive chapters contains detailed protocols and a short set of primary references. ... there is sufficient detail to allow direct adaptation in the laboratory. ... the volume contains useful material for investigators working in this area." (Eugene A Davidson, Doody's Book Review Service, March, 2006)
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