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Mast Cells : Methods and Protocols

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Description

This is a cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells. These methods take advantage of the latest advances in molecular biology, technology, and information science. They include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis.show more

Product details

  • Hardback | 439 pages
  • 156 x 236 x 26mm | 879.98g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2005 ed.
  • 115 black & white illustrations, biography
  • 1588293742
  • 9781588293749

Review quote

From the reviews: "This volume provides a set of protocols for investigators working with mast cells. Each of the chapters is accompanied by a short bibliography. ... The information will be of value to advanced students and laboratory investigators. ... Each of the substantive chapters contains detailed protocols and a short set of primary references. ... there is sufficient detail to allow direct adaptation in the laboratory. ... the volume contains useful material for investigators working in this area." (Eugene A Davidson, Doody's Book Review Service, March, 2006)show more

Back cover copy

The mast cell, long implicated in causing allergic reactions, may also be involved in many other disease processes, including cancer, heart disease, parasitic disease, atherosclerosis, asthma, and arthritis. In Mast Cells: Methods and Protocols, hands-on experts describe in detail their best techniques for the isolation, culture, and study of both activation and signaling in human mast cells. These readily reproducible methods take advantage of the latest advances in molecular biology, technology, and information science. The techniques provide a sound base of methodology for mast cell research and include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis. A survey of mast cell biology offers insight into its history and the implications for adaptive immunity. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Mast Cells: Methods and Protocols provides mast cell researchers with reproducible accounts of basic and advanced molecular and cellular techniques used in studying this fascinating, multifunctional cell."show more

Table of contents

Part I. Overview of Mast Cell Biology Paul Ehrlich's 'Mastzellen'-From Aniline Dyes to DNA Chip Arrays: A Historical Review of Developments in Mast Cell Research Harsha Vyas and Guha Krishnaswamy The Human Mast Cell: An Overview Guha Krishnaswamy, Omar Ajitawi, and David S. Chi Mast Cells in Allergy and Autoimmunity: Implications for Adaptive Immunity Gregory D. Gregory and Melissa A. Brown Part II. Identification of Mast Cells in Culture and in Tissue Analysis of MCT and MCTC Mast Cells in Tissue Lawrence B. Schwartz Mast Cell Ultrastructure and Staining in Tissue Shruti A. Shukla, Ranjitha Veerappan, Judy S. Whittimore, Lou Ellen Miller, and George A. Youngberg Expression of Cell Surface Antigens on Mast Cells: Mast Cell Phenotyping Alexander W. Hauswirth, Stefan Florian, Gerit-Holger Schernthaner, Maria-Theresa Krauth, Karoline Sonneck, Wolfgang R. Sperr, and Peter Valent Identification of Mast Cells in the Cellular Response to Myocardial Infarction Nikolaos G. Frangogiannis and Mark L. Entman Part III. Development of Mast Cells In Vitro Growth of Human Mast Cells From Bone Marrow and Peripheral Blood-Derived CD34+ Pluripotent Progenitor Cells Arnold S. Kirshenbaum and Dean D. Metcalfe Culture of Human Mast Cells From Hemopoietic Progenitors Hirohisa Saito Isolation, Culture, and Characterization of Intestinal Mast Cells Gernot Sellge and Stephan C. Bischoff Part IV. Mast Cell Signaling and Gene Expression Activation of Nuclear Factor-kB Chuanfu Li, Jim Kelley, and Tuanzhu Ha Analysis of Mitogen-Activated Protein Kinase Activation Stephen C. Armstrong Microarray and Gene-Clustering Analysis Tsung-Hsien Tsai, Denise M. Milhorn, and Shau-Ku Huang Techniques to Study FceRI Signaling Yuko Kawakami, Jiro Kitaura, and Toshiaki Kawakami Part V. Mast Cell Expression and Release of Inflammatory Mediators Human Mast Cell Proteases: Activity Assays Using Thiobenzyl Ester Substrates David A. Johnson Mast Cell Histamine and Cytokine Assays David S. Chi, S. Matthew Fitzgerald, and Guha Krishnaswamy Measurement of Mast Cell Cytokine Release by Multiplex Assay Kevin F. Breuel and W. Keith De Ponti Assays for Histamine-Releasing Factors: From Identification and Cloning to Discovery of Binding Partners Jacqueline M. Langdon and Susan M. MacDonald Assays for Nitric Oxide Expression William L. Stone, Hongsong Yang, and Min Qui Immunohistological Detection of Growth Factors and Cytokines in Tissue Mast Cells Zhenhong Qu Part VI. Mast Cell Interactions With Other Cell Types Endothelial Cell Activation by Mast Cell Mediators Kottarappat N. Dileepan and Daniel J. Stechschulte Co-Culture of Mast Cells With Fibroblasts: A Tool to Study Their Crosstalk Ido Bachelet, Ariel Munitz, and Francesca Levi-Schaffer Detection of e Class Switching and IgE Synthesis in Human B Cells Jerome Pene, Florence Guilhot, Isabelle Cognet, Paul Guglielmi, Angelique Guay-Giroux, Jean-Yves Bonnefoy, Greg C. Elson, Hans Yssel, and Jean-Francois Gauchat Part VII. Novel Aspects of Mast Cell Activation and Regulation Gene Silencing Using Small Interference RNA in Mast Cells Deling Yin and Charles A. Stuart Mast Cell Activation by Lipoproteins Jim Kelley, Gregory Hemontolor, Walid Younis, Chuanfu Li, Guha Krishnaswamy, and David S. Chi Mast Cell Activation by Stress Ann L. Baldwin Part VIII. Roles of Mast Cells in Host Defense In Vivo Models for Studying Mast Cell-Dependent Responses to Bacterial Infection Christopher P. Shelburne, James B. McLachlan, and Soman N.show more

Review Text

From the reviews: "This volume provides a set of protocols for investigators working with mast cells. Each of the chapters is accompanied by a short bibliography. ... The information will be of value to advanced students and laboratory investigators. ... Each of the substantive chapters contains detailed protocols and a short set of primary references. ... there is sufficient detail to allow direct adaptation in the laboratory. ... the volume contains useful material for investigators working in this area." (Eugene A Davidson, Doody's Book Review Service, March, 2006)show more