Mammalian Synthetic Promoters
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Mammalian Synthetic Promoters

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Description

This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and cutting-edge, Mammalian Synthetic Promoters: Methods and Protocols aims to aid researchers that are new to the field of synthetic promoters and inspire new developments.
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Product details

  • Paperback | 277 pages
  • 178 x 254 x 15.49mm | 559g
  • Totowa, NJ, United States
  • English
  • Softcover reprint of the original 1st ed. 2017
  • 25 Illustrations, color; 18 Illustrations, black and white; XI, 277 p. 43 illus., 25 illus. in color.
  • 1493984217
  • 9781493984213

Back cover copy

This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mammalian Synthetic Promoters: Methods and Protocols aims to aid researchers that are new to the field of synthetic promoters and inspire new developments.
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Table of contents

1. Initial Considerations before Designing a Promoter Construct David Gould







Part I: Monitoring Promoter Interactions and Activity



2 Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay



Nasim Yousaf and David Gould







3. Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter



Jordan Read







4. Secreted Reporters for Monitoring Multiple Promoter Function



Ghazal Lashgari, Rami S. Kantar, and Bakhos A. Tannous







5. Bioluminescence Monitoring Of Promoter Activity In Vitro and In Vivo



Juliette MKM Delhove, Rajvinder Karda, Kate E Hawkins, Simon N Waddington, and Tristan R McKay







6. Monitoring Promoter Activity by Flow Cytometry



Taher E. I. Taher







Part II: Promoter Design and Applications



7. Functional Screening of Core Promoter Activity



Dan Y. Even, Adi Kedmi, Diana Ideses, and Tamar Juven-Gershon







8. Bioinformatically Informed Design of Synthetic Mammalian Promoters



Michael L. Roberts, Polyxeni Katsoupi, Vivian Tsevleki, and Era Taoufik







9. Synthetic Tumor Specific Promoters for Transcriptional Regulation of Viral Replication



M. Veronica Lopez, Eduardo. G. Cafferata, Diego. L. Viale, and Osvaldo Podhajcer







10. Constructing Strong Cell-Type-Specific Promoters through Informed Design



Adam J. Brown and David C. James







11. PCR Assembly of Synthetic Promoters



Hodan Mohamed and David Gould







Part III: Regulated Promoter Approaches



12. The Tetracycline Responsive System



Nasim Yousaf and David Gould







13. Light-responsive Promoters



Maximilian Hoerner, Konrad Muller, and Wilfried Weber







14. A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation



Ryohei Ogawa, Go Kagiya, Akihiko Watanabe, Akihiro Morii, Zheng-Guo Cui, and Takashi Kondo



15. Promoter Activation with Electromagnetism



Abraham O. Rodriguez-De la Fuente, J. Antonio Heredia-Rojas, Juan M. Alcocer-Gonzalez, Laura E. Rodriguez-Flores, Cristina Rodriguez-Padilla, and Reyes S. Tamez-Guerra







16. Application of Synthetic Tumor Specific Promoters Responsive to the Tumor Microenvironment



Eduardo G Cafferata, M Veronica Lopez, Felipe J Nunez, Maria Agostina Russo Maenza, and Osvaldo L Podhajcer







Part IV: Combining Promoters in Synthetic Circuits



17. A Modular Approach to Building Complex Synthetic Circuits



Yinqing Li and Ron Weiss







18. Computational Sequence Design with R2oDNA Designer



James T. MacDonald and Velia Siciliano







19. Design of Synthetic Promoters for Gene Circuits in Mammalian Cells



Pratik Saxena, Daniel Bojar, and Martin Fussenegger
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