Macromolecular Crystallography Protocols, Volume 1
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Macromolecular Crystallography Protocols, Volume 1 : Preparation and Crystallization of Macromolecules

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Description

Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization. The volume concludes with a survey of available crystallographic software.
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Product details

  • Hardback | 280 pages
  • 157.5 x 228.6 x 20.3mm | 453.6g
  • Totowa, NJ, United States
  • English
  • 2007 ed.
  • 49 Illustrations, black and white; XIV, 280 p. 49 illus.
  • 1588292924
  • 9781588292926

Table of contents

TABLE OF CONTENTS VOLUME 1

HUMANA PRESS-CRYSTALLOGRAPHIC METHODS AND PROTOCOLS
Sylvie Doublie, Editor

CHAPTER TITLE AUTHOR(S) PAGE

1.
A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual His6-MBP Affinity Tag
Joseph E. Tropea, Scott Cherry, Sreedevi Nallamsetty, Christophe Bignon, and David S.Waugh

1-29
2. Cloning, production, and purification of proteins for a medium scale structural genomics project Sophie Quevillon-Cheruel, Bruno Collinet, Lionel Tresaugues, Philippe Minard, Gilles Henckes, Robert Aufrere, Karine Blondeau, Cong-Zhao Zhou, Dominique Liger, Nabila Bettache, Anne Poupon, Ilham Aboulfath, Nicolas Leulliot, Joel Janin, and Herman van Tilbeurgh




1-26
3. Baculoviral expression of an integral membrane protein for structural studies
Dean R. Madden and Markus Safferling 1-33
4. Protein Engineering Sonia Longhi, Francois Ferron, and Marie-Pierre Egloff

1-48
5. Selenomethionyl protein expression Sylvie Doublie 1-21

6. How to Use Dynamic Light Scattering to Improve Your Chances of Growing Crystals of a Macromolecule
Gloria Borgstahl 1-34
7. Screening and optimization methods for non-automated crystallization laboratories
Terese Bergfors 1-32
8. Improving Marginal Crystals Charles W. Carter, Jr. and
Madeleine Ries-Kautt

1-34
9. Optimisation Techniques for Automation and High Throughput
Naomi E. Chayen 1-26
10. Three-dimensional crystallization of membrane proteins

James Fethiere 1-40
11. Crystallization of Protein-DNA complexes
Thomas Hollis 1-20
12. Preparation and crystallization of RNA
Barbara Golden 1-24
13. Crystallisation of RNA-protein complexes Eiji Obayashi, Chris Oubridge, Daniel Pomeranz Krummel, and Kiyoshi Nagai
1-27
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