Macromolecular Crystallography Protocols: v. 1

Macromolecular Crystallography Protocols: v. 1 : Preparation and Crystallization of Macromolecules

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Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization. The volume concludes with a survey of available crystallographic more

Product details

  • Hardback | 280 pages
  • 157.5 x 228.6 x 20.3mm | 453.6g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 49 black & white illustrations, biography
  • 1588292924
  • 9781588292926

Table of contents

TABLE OF CONTENTS VOLUME 1 HUMANA PRESS-CRYSTALLOGRAPHIC METHODS AND PROTOCOLS Sylvie Doublie, Editor CHAPTER TITLE AUTHOR(S) PAGE 1. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual His6-MBP Affinity Tag Joseph E. Tropea, Scott Cherry, Sreedevi Nallamsetty, Christophe Bignon, and David S.Waugh 1-29 2. Cloning, production, and purification of proteins for a medium scale structural genomics project Sophie Quevillon-Cheruel, Bruno Collinet, Lionel Tresaugues, Philippe Minard, Gilles Henckes, Robert Aufrere, Karine Blondeau, Cong-Zhao Zhou, Dominique Liger, Nabila Bettache, Anne Poupon, Ilham Aboulfath, Nicolas Leulliot, Joel Janin, and Herman van Tilbeurgh 1-26 3. Baculoviral expression of an integral membrane protein for structural studies Dean R. Madden and Markus Safferling 1-33 4. Protein Engineering Sonia Longhi, Francois Ferron, and Marie-Pierre Egloff 1-48 5. Selenomethionyl protein expression Sylvie Doublie 1-21 6. How to Use Dynamic Light Scattering to Improve Your Chances of Growing Crystals of a Macromolecule Gloria Borgstahl 1-34 7. Screening and optimization methods for non-automated crystallization laboratories Terese Bergfors 1-32 8. Improving Marginal Crystals Charles W. Carter, Jr. and Madeleine Ries-Kautt 1-34 9. Optimisation Techniques for Automation and High Throughput Naomi E. Chayen 1-26 10. Three-dimensional crystallization of membrane proteins James Fethiere 1-40 11. Crystallization of Protein-DNA complexes Thomas Hollis 1-20 12. Preparation and crystallization of RNA Barbara Golden 1-24 13. Crystallisation of RNA-protein complexes Eiji Obayashi, Chris Oubridge, Daniel Pomeranz Krummel, and Kiyoshi Nagai 1-27show more