Lysosomes and Lysosomal Diseases: Volume 126

Lysosomes and Lysosomal Diseases: Volume 126

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This new volume of Methods in Cell Biology looks at methods for lysosomes and lysosomal diseases. Chapters focus upon practical experimental protocols to guide researchers through the analysis of multiple aspects of lysosome biology and function. In addition, it details protocols relevant to clinical monitoring of patients with lysosomal diseases. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.
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Product details

  • Hardback | 450 pages
  • 191 x 235 x 25.4mm | 1,180g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0128000791
  • 9780128000793
  • 2,173,143

Table of contents

1. Methods for Monitoring Lysosomal Morphology
Bethan S. Kilpatrick, Emily R. Eden, Leanne N. Hockey, Clare E. Futter and Sandip Patel
2. A Rapid Method for the Preparation of Ultra-pure, Functional Lysosomes Using Funcionalised Superparamagnetic Iron Oxide Nanoparticles
Mathew William Walker and Emyr Lloyd-Evans
3. TFEB and the CLEAR Network
Carmine Settembre and Diego L. Medina
4. Biosynthesis, Targeting and Processing of Lysosomal Proteins: Pulse-Chase Labeling and Immune Precipitation
Sandra Pohl and Andrej Hasilik
5. Measuring Lysosomal pH by Fluorescence Microscopy
Johnathan Canton and Sergio Grinstein
6. Lysosome Fusion in Cultured Mammalian Cells
Nicholas A. Bright, Lena Wartosch and J. Paul Luzio
7. RNAi Screens of Lysosomal Trafficking
Salil Garg, Michael B. Brenner
8. Approaches for Plasma Membrane Wounding and Assessment of Lysosome-mediated Repair Responses
Corrotte, M, Castro-Gomes, T, Koushik, A.B. and Andrews, N.W
9. Imaging Approaches to Measuring Lysosomal Calcium
Anthony J. Morgan, Lianne C. Davis, Antony Galione
10. Lysosome Electrophysiology
Xi Zoe Zhong and Xian-Ping Dong
11. Reconstitution of Lysosomal Ion Channels into Artificial Membranes
Elisa Venturi and Rebecca Sitsapesan
12. Fluorescence Methods for Analysis of Interactions Between Ca2+ Signalling, Lysosomes and Endoplasmic Reticulum
David L. Prole, Cristina I. Lopez-Sanjurjo, Stephen C. Tovey and Colin W. Taylor
13. Methods for the Quantification of Lysosomal Membrane Permeabilization: a Hallmark of Lysosomal Cell Death
Sonja Aits, Marja Jaattela and Jesper Nylandsted
14. Measuring the Phagocytic Activity of Cells
Nick Platt and Paul Fineran
15. Detection and Quantification of Microbial Manipulation of Phagosomal Function
Maria Podinovskaia, David G. Russell
16. Measuring Relative Lysosomal Volume for Monitoring Lysosomal Storage Diseases
Danielle te Vruchte, Kerri L. Wallom and Frances M. Platt
17. Quantifying Storage Material Accumulation in Tissue Sections
Jonathan D Cooper, Helen R Brooks, Hemanth R Nelvagal
18. Laboratory Diagnosis of Niemann-Pick disease Type C: The Filipin Staining Test
Marie T. Vanier and Philippe Latour
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Review quote

Praise for the Series: "The series is invaluable for workers at all levels of cell biology." --Nature
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About Nicky Platt

Prof. Frances Platt obtained her PhD from the University of Bath, UK, and was a post-doctoral fellow at Washington University Medical School in St. Louis, USA. She was a Lister Institute Senior Research Fellow and a Reader at the University of Oxford. Prof. Platt's main research interests include the biology and pathobiology of glycosphingolipids. Her research led to the development of miglustat for the treatment of glycosphingolipid storage diseases. In 1999, Prof. Platt was awarded the Alan Gordon Memorial Award and the Horst Bickel Award for advances in metabolic disease therapy. She was elected a fellow of the Academy of Medical Sciences in 2011. Dr Platt obtained his PhD from the University of Bath, UK and was a post-doctoral fellow at Washington University in St Louis. Since his return to UK he has worked at University of Oxford in the Sir William Dunn School of Pathology and Nuffield Department of Medicine. He has very recently become a senior research fellow in the Department of Pharmacology. His research interests are in innate immunity, particularly the role of specific pattern recognition receptors in immune and inflammatory responses.
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