Lymphoma

Lymphoma

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A cutting-edge collection of readily reproducible molecular techniques to better understand, classify, and treat lymphoma. Among the highlights are methods to use immunoglobulin gene rearrangements as markers of clonality, to exploit patterns of somatic mutation in the variable regions to indicate at which stage transformation occurred, and to apply gene arrays to the question of biological heterogeneity in morphologically similar diseases. Research methodologies that are highly likely to become routine practice in the future, such as DNA microarray and immunoglobulin V-gene rearrangements, and measurement of minimal disease, are included. There are also molecular techniques for providing for producing novel therapeutics, such as a DNA vaccine with patient-specific sequences derived from the lymphoma in question.
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Product details

  • Hardback | 342 pages
  • 156 x 232 x 26mm | 698.54g
  • Totowa, NJ, United States
  • English
  • 2005 ed.
  • 43 Illustrations, black and white; XVIII, 342 p. 43 illus.
  • 1588291596
  • 9781588291592

Back cover copy

The tremendous progress made over the last few years in our molecular understanding of lymphocyte biology and lymphoma classification promises to have a major impact on clinical practice. In Lymphoma: Methods and Protocols, leading clinical investigators from world renowned laboratories describe in step-by-step detail the latest molecular techniques being used to better understand, classify, and treat lymphoma. Among the highlights are methods to use immunoglobulin gene rearrangements as markers of clonality, to exploit patterns of somatic mutation in the variable regions to indicate at which stage transformation occurred, and to apply gene arrays to the question of biological heterogeneity in morphologically similar diseases. Research methodologies that are highly likely to become routine practice in the future, such as DNA microarray and immunoglobulin V-gene rearrangements, and measurement of minimal disease, are included. There are also molecular techniques for producing novel therapeutics, such as a DNA vaccine with patient-specific sequences derived from the lymphoma in question. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Lymphoma: Methods and Protocols offers oncologists, hematologists, and clinical scientists a diverse collection of readily reproducible techniques for the molecular diagnosis and prognostic evaluation of lymphomas.
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Table of contents

Purification of Primary Malignant B-Cells and Immunoblot Analysis of Bcl-2 Family Proteins
Claire Dallman and Graham Packham

Molecular Diagnosis of Lymphoma: Outcome Prediction by Gene Expression Profiling in Diffuse Large B-Cell Lymphoma
Kim Last, Silvana Debarnardi, and T. Andrew Lister

Demonstration of a Germinal Center Immunophenotype in Lymphomas by Immunocytochemistry and Flow Cytometry
Andrew Jack, Sharon Barrans, David Blythe, and Andrew Rawstron

Karyotyping Lymph Node Biopsies in Non-Hodgkin's Lymphoma
Fiona M. Ross and Christine J. Harrison

Identification of Lymphoma-Associated Antigens Using SEREX
Amanda P. Liggins, Barbara A. Guinn, and Alison H. Banham

Determining Mutational Status of Immunoglobulin V Genes in Chronic Lymphocytic Leukemia: A Useful Prognostic Indicator
Surinder S. Sahota, Gavin Babbage, Niklas Zojer, Christian H. Ottensmeier, and Freda K. Stevenson

Idiotype Gene Rescue in Follicular Lymphoma
Katy McCann, Surinder S. Sahota, Freda K. Stevenson, and Christian H. Ottensmeier

Immunoglobulin Gene Mutation Patterns and Heterogeneity of Marginal Zone Lymphoma
Mariella Dono and Manlio Ferrarini

T-Cell Receptor Molecular Diagnosis of T-Cell Lymphoma
Elizabeth Hodges, Anthony P. Williams, Susan Harris, and John L. Smith

Cloning of Immunoglobulin Chromosomal Translocations by Long-Distance Inverse Polymerase Chain Reaction
E. Loraine Karran, Takashi Sonoki, and Martin J. S. Dyer

Sequential Fluorescence In Situ Hybridization Analysis for Trisomy 12 in B-Cell Chronic Lymphocytic Leukemia
Viktoria Hjalmar

Splenic Marginal Zone Lymphoma: 7q Abnormalities
Rachel E. Ibbotson, Anton E. Parker, and David G. Oscier

BCL-6: Rearrangement and Mutation in Lymphoma
Simon D. Wagner and Jaspal S. Kaeda

Antibody Techniques Used in the Study of Anaplastic Lymphoma Kinase-Positive ALCL
Karen Pulford, Helen M. Roberton, and Margaret Jones

Identification of Anaplastic Lymphoma Kinase Variant Translocations Using 5'RACE
Luis Hernandez and Elias Campo

Follicular Lymphoma: Quantitation of Minimal Residual Disease by PCR of the t(14;18) Translocation
Angela Darby and John G. Gribben

Index
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Review quote

"Offers(s) a diverse collection of readily reproducible techniques for the molecular diagnosis and prognostic evaluation of lymphomas..." - Clinical Laboratory International
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