Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate: Volume 533

Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate: Volume 533 : Cell, Lipid and Carbohydrates

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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.
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Product details

  • Hardback | 408 pages
  • 152.4 x 231.14 x 25.4mm | 748.42g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0124200672
  • 9780124200678

Table of contents

Pouring Agar Plates and Streaking or Spreading to Isolate Individual Colonies

Storage of Bacteria and Yeast

Cellular Fractionation - Mammalian Cells

Cellular Fractionation - Yeast cells

Yeast-Gene Replacement Using PCR Products

Measuring RNAi knockdown using qPCR

Recombineering: Using Drug Cassettes to Knock out Genes in vivo

Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae

Gene targeting and Site-specific recombination in Mouse ES Cells

Recombineering: Highly Efficient in vivo genetic engineering using single-strand oligos

Growth Media for E. coli

Saccharomyces cerevisiae Growth Media

Immunohistochemistry on Freely Floating Fixed Tissue Sections

Preparation of Slides for Microscopy from Frozen Tissue Sections

Preparation of Formalin-fixed Paraffin-embedded Tissue for Immunohistochemistry

Preparation of Cells for Microscopy using Cytospin

Preparation of Cells for Microscopy using Chamber Slides and Coverslips

Preparation of Cells for Microscopy using `Cell Blocks'

Enzymatic deglycosylation of glycoproteins

Preparation of fatty acid or phospholipid vesicles by thin-film rehydration

Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder

Preparation of fatty acid micelles

Reverse-phase HPLC Analysis and Purification of Small Molecules

Thin Layer Chromatography

ATP and GTP hydrolysis assays (TLC)
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