Laboratory Methods in Enzymology: RNA: Volume 530

Laboratory Methods in Enzymology: RNA: Volume 530 : RNA

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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.
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Product details

  • Hardback | 472 pages
  • 154.94 x 231.14 x 27.94mm | 861.82g
  • Academic Press Inc
  • San Diego, United States
  • English
  • Illustrations (black and white)
  • 0124200370
  • 9780124200371

Table of contents

Computational Prediction of RNA Secondary Structure

Reverse-transcription PCR (RT-PCR)

Northern blotting

Explanatory Chapter: Nuclease Protection Assays

In Vitro Transcription from Plasmid or PCR-amplified DNA

Measurement of In Vivo RNA Synthesis Rates

Method for measuring mRNA decay rate in Saccharomyces cerevisiae

Analysis of polysomes from bacteria

Polysome Profile Analysis - Yeast

Polysome analysis of mammalian cells

Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae

Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates

Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3'; ends, and poly(A) tails of RNA

RNA Radiolabeling

Fluorescently Labeling Synthetic RNAs

Analysis of RNA by analytical polyacrylamide gel electrophoresis

RNA purification by preparative polyacrylamide gel electrophoresis

Explanatory Chapter: Nucleic Acid Concentration Determination

RNA purification - precipitation methods

Reverse Transcriptase Dideoxy Sequencing of RNA

RNA Structure Experimental Analysis - Chemical Modification

Structural Analysis of RNA Backbone Using In-Line Probing
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