The Kansas University Science Bulletin Volume 10, Nos. 1-15

The Kansas University Science Bulletin Volume 10, Nos. 1-15

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This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1917 edition. Excerpt: ...others were crushed for studies of living sporocysts and rediae. The fixatives used were a saturated corrosive sublimate solution with one per cent glacial acetic acid, Zenker's fluid, and four per cent formalin. The latter was discarded after trials proved the superiority of the other fixatives. Corrosive sublimate was preferable for free cercariae. Several different stains were used. Picrocarmine was excellent for studies of the living cercariae. Specimens so stained could sometimes be preserved by drawing glycerine under the cover slip with filter paper. For toto counts, Mayer's haemalum gave the best results. The sections were cut from five to seven micra in thickness and stained with iron alum haematoxylin. Good results were also obtained by staining with haemalum and using eosin or orange g. for counter stains. The free cercariae could be made to expand by heating slightly. When they were in this condition the fixing fluid was poured on suddenly, fixing the specimens in the best form for study. The measurements given in this paper were taken usually from mounted specimens, and are not as accurate as those from living forms would be, because of shrinkage of the specimens during the process of fixing and mounting. Amphistome Cercarle. Three species of amphistome cercariae were found in my collections. For the first of these I propose the name Cercaria cortii. Of twenty-three specimens of Planorbis trivolvis collected at Cherryvale, Kan., October 16, one was infected with Cercaria cortii. The snails were found adhering to rocks at a depth of from six inches to a foot in a large pond of clear water. On October 17 the snails were isolated in individual watch glasses and kept covered with water, the water being changed each day. more

Product details

  • Paperback
  • 189 x 246 x 3mm | 132g
  • United States
  • English
  • black & white illustrations
  • 1236813790
  • 9781236813794