Johns Hopkins Hospital Bulletin Volume 18

Johns Hopkins Hospital Bulletin Volume 18

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This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1907 edition. Excerpt: ...of this fact, it must be expected that a comparative count of phagocyted typhoid bacilli can not be attempted without considerable error. One might hope to reduce these errors by a shorter incubation time. However, that would not be advisable in the determination of the opsonic index, since due to slight differences in the diameter of the capillary pipettes, unequal heating would follow if the incubation time were too short. In order to avoid these disadvantages, it was very natural to apply an analogous method to that used for the determination of agglutinins, namely, to determine in what degree of dilution the serum ceases to cause phagocytosis. By this method very definite results were obtained. Of course, as in all absolute measurements, some precautions must be carefully observed. First, the choice of the test-bacillus is of great importance. As shown by Lohlein," the individual qualities of a given strain are of marked influence on the resulting phagocytosis, and on the action of opsonins. Lohlein, for instance, found among three different strains of B. coli one that was opsonized very readily; the other two were but little influenced by normal serum. One of them was very susceptible to phagocytosis, without any opsonic action by the serum; the other one was not taken up at all, even under the influence of serum. According to Lohlein the virulent bacteria generally have the greatest resistance to phagocytosis. In order to get good results with the above-mentioned dilution method, it is best to use a strain which is not susceptible to phagocytosis without preparation by opsonins. The typhoid strain used by me for the opsonin test was of this kind. It was grown directly from the blood of a typhoid patient. In normal more

Product details

  • Paperback | 472 pages
  • 189 x 246 x 24mm | 835g
  • United States
  • English
  • black & white illustrations
  • 1236758803
  • 9781236758804