Immunology Methods Manual, Four-Volume Set

Immunology Methods Manual, Four-Volume Set : The Comprehensive Sourcebook of Techniques

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The Immunology Methods Manual is a four-volume set comprising a comprehensive sourcebook of immunological methods and techniques. Unique in its project-oriented, rather than protocol-oriented approach, the emphasis is placed on describing the environment in which each method was developed and is used. The manual contains both advanced methods and descriptions and basic protocols. It is essential not only for those who study the immune system of man or mouse, but also those applying methods to other species. This new compilation of methods answers the needs of immunologists; molecular, developmental, and cell biologists; students and advanced researchers, and workers in clinical and diagnostic laboratories. Protocols are provided to guide day-to-day laboratory work as well as the development of long-term strategies. Vital contact information also encourages useful dialogue and interaction between authors and method-users.
More than 35 section editors and 350 authors have contributed to compile this four-volume practical guide to methods that work!
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Product details

  • Mixed media product | 2495 pages
  • 181.9 x 233.2 x 18.5mm | 530.71g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0124427103
  • 9780124427105

Review quote

"An invaluable contribution to the work of researchers engaged in state of the art immunological research."
"Ivan Lefkovits is one of the pioneers in Immunological Technology. That he has now put together a book on this subject is very exciting and should proves useful to many workers in the field."
"With this crew, it will fly high."
"This volume is of exceptional value because it draws on the broad experience of the Basel Institute of Immunology... It will be used by immunologists at every level, from beginners to old hands, and will be cited as the definitive reference for many techniques."
"Edited by a master of immunological techniques, Dr. Ivan Lefkovits, this authoritative work fully meets its ambitious goals. The wealth of information provided in the Manual should help not only graduate students and postdoctoral fellows in immunology, but more experienced research workers and laboratory scientists as well."
"I congratulate you on this novel endeavor in compiling cutting edge technology in immunological research. It represents a most timely effort, as a comprehensive up-to-date methods manual has not been available for many years. The list of contributors is certainly impressive. A must for all actively experimenting immunologists."
"This manual has a comprehensive set of advanced immunological techniques and is particularly strong in cellular immunology. The price/page ratio will make the manual widely accessible to research laboratories."
The Prepublication Reviews
"Ivan Lefkovits is one of the pioneers in Immunological Technology. That he has now put together a book on this subject is very exciting and should prove useful to many workers in the field."
"This manual consists of a four-volume set that draws on the expertise of more than 300 researchers. Despite the large number of authors, the style of presentation is surprisingly uniform, there is minimal repetition, and the illustrations are clear and effective... Overall, the manual is well produced, and it is an especially good source of methods in cellular Immunology.
--Daniel Quinn, PhD, Loyola University Stritch School of Medicine, for DOODY'S PUBLISHING REVIEWS
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Table of contents

Volumes 1-4: Section Headings & Editors:

Volume 1:
A. Traunecker, Expression of Recombinant Proteins.
J. Adair, Engineered Molecules.
M. Kopf, Gene Targeting.
G.E. Wu, Monitoring V(D)J Rearrangement.
M.V. Wiles, The Art of Polymerase Chain Reaction (PCR).
H.-J. Thiesen, Transcriptional Gene Regulation in B and T Cells.
J.A. Garcia-Sanz, mRNA Expression.
R. Nezlin, Antigen, Antibody and Immune Complexes.

Volume 2:
H-G. Rammensee, MHC Ligands and Peptide Binding.
S. Weiss, Screening of T and B Cell Epitopes.
C.J. Paige, Development of Cells of the B Lineage.
A. Rolink, Ex vivo and in vitro Methods to Study B Lymphopoiesis in Mouse and Man.
R. Benner, Modulation of the Humoral Immune Response and Its Measurement.
C. Martinez-A., Signaling Pathways and Lymphocyte Cell Repertoires.

Volume 3:
K.-U. Hartmann, Immunohistological Tools.
F. McConnell, Lymphocytes as Single Cells.
J. Quintans, Lymphoid Lines as Models for Physiological Cell Death.
B.A. Imhof, Leukocyte Migration and Adhesion.
S.A. Berger, Mast Cells.
M.H. Kosco-Vilbois, Dendritic Cells of Mice and Humans.
A. Augustin and G.-K. Sim, Isolation of Lymphocytes from Non-Lymphoid Tissues.
H. Tlaskalova-Hogenova and J. Sterzl, Gnotobiological Models.
M.P. Hazenberg, Interaction of Peptidoglycan-polysaccharides from the Human Intestinal Flora Bacteria and the Immune System.
A. Toivanen and P. Toivanen, Rheumatology in Relation to Immune Response and Microbes.
H. Wekerle, Experimental Models of Organ-specific Autoimmunity (Nervous System).
L. Adorini, Experimental Models of Autoimmune Diseases.

Volume 4:
J.J.M. van Dongen, Immunodiagnosis of B-Cell Malignancies.
R. Zinkernagel and H. Hengartner, Immune Responses Against VSV, LCMV and Vaccinia Virus In Vivo and In Vitro.
J.L. Hurwitz, HIV and the Immune Response.
W.R. Hein, In Vivo Techniques of Experimental Immunology in Sheep.
W.R. Hein and P. Griebel, In Vitro Techniques of Experimental Immunology in Sheep.
O. Vainio, Experimental Immunology in Chickens.
L. Du Pasquier, Cold-Blooded Vertebrates.
J.J.M. van Dongen and P.J.M. Leenan, Leukocyte Markers and the CD Nomenclature.
Subject Index included in each volume.
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