Immunogenetics
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Immunogenetics : Methods and Applications in Clinical Practice

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Description

The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells. They are also the major histocompatibility barriers to transplantation, which is the clinical paradigm of the self versus non self concept. It is now recognized that this diverse range of gene systems involved in the control of the immune response have been shown to be important in many aspects of clinical practice. As a result many new molecular and cellular methods have been developed for identifying these genes and their polymorphisms, and immunogenetic laboratories specializing in these methods have developed to support transplantation and other clinical programs. Immunogenetics: Methods and Applications in Clinical Practice focuses on methods for human clinical practice. The emphasis rests on those assays which are of established or potential clinical utility and are likely to be included in the repertoire of tests provided by a routine diagnostic and service laboratory. This volume also contains several review chapters of the MHC complex, the KIR complex, the human immunoglobulin allotypes, as well as reviews of the methods for the detection of alloreactive NK cells and the detection of HLA antibodies by solid phase assays. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.



Authoritative and easily accessible, Immunogenetics: Methods and Applications in Clinical Practice seeks to serve both the immunogenetics community and the wider scientific community with a collection of detailed information and helpful tips attained by many years of experience in the field.
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Product details

  • Hardback | 689 pages
  • 180.34 x 256.54 x 45.72mm | 1,428.81g
  • Totowa, NJ, United States
  • English
  • 2012 ed.
  • 98 Tables, black and white; 2 Illustrations, color; 135 Illustrations, black and white; XVI, 689 p. 137 illus., 2 illus. in color.
  • 161779841X
  • 9781617798412
  • 1,136,967

Back cover copy

The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells. They are also the major histocompatibilty barriers to transplantation, which is the clinical paradigm of the self versus non self concept. It is now recognized that this diverse range of gene systems involved in the control of the immune response have been shown to be important in many aspects of clinical practice. As a result many new molecular and cellular methods have been developed for identifying these genes and their polymorphisms, and immunogenetic laboratories specializing in these methods have developed to support transplantation and other clinical programs. Immunogenetics: Methods and Applications in Clinical Practice focuses on methods for human clinical practice. The emphasis rests on those assays which are of established or potential clinical utility and are likely to be included in the repertoire of tests provided by a routine diagnostic and service laboratory. This volume also contains several review chapters of the MHC complex, the KIR complex, the human immunoglobulin allotypes, as well as reviews of the methods for the detection of alloreactive NK cells and the detection of HLA antibodies by solid phase assays. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.



Authoritative and easily accessible, Immunogenetics: Methods and Applications in Clinical Practice seeks to serve both the immunogenetics community and the wider scientific community with a collection of detailed information and helpful tips attained by many years of experience in the field.
show more

Table of contents

1. The Major Histocompatibility Complex - A Paradigm for Studies of the Human Genome

Richard J. N. Allcock



2. HLA Typing by SSO and SSP Methods

Heather Dunckley



3. Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity

Michael D. Varney, Alison S. L. Castley, Katri Haimila and Paivi Saavalainen



4. HLA Typing Using Bead Based Methods

Daniel Trajanoski and Samantha J. Fidler



5. HLA Typing by Direct DNA Sequencing

Linda K. Smith



6. Data Analysis of HLA Sequencing using Assign-SBT v3.6+ from Conexio

Carla Wirtz and David Sayer



7. Simple Methods for the Detection of HLA-G Variants in Coding and Non-Coding Regions

Holger Nuckel, Erick C. Castelli, Philippe Moreau, Crista Ochsenfarth, Peter A. Horn, and Vera Rebmann



8. Molecular Typing of HLA-E

Nina Lauterbach, Christina E.M. Voorter, and Marcel G.J. Tilanus



9. Molecular Analysis of Complement Component C4 Gene Copy Number

Alison Castley and Olga Patricia Martinez



10. Genotyping of Single Nucleotide Polymorphisms (SNPs) by 5' Nuclease Allelic Discrimination

Mari Malkki and Effie W. Petersdorf



11. High Resolution MICA Genotyping by Sequence-Based Typing (SBT)

Yizhou Zou and Peter Stastny



12. Standard Methods for the Management of Immunogenetic Data

Pierre-Antoine Gourraud, Jill A. Hollenbach, Thomas Barnetche, Richard M. Single, and Steven J. Mack



13. Analytical Methods for Immunogenetic Population Data

Steven J. Mack, Pierre-Antoine Gourraud, Richard M. Single, Glenys Thomson, and Jill A. Hollenbach



14. Analytical Methods for Disease Association Studies with Immunogenetic Data

Jill A. Hollenbach, Steven J. Mack, Glenys Thomson, and Pierre-Antoine Gourraud



15. Impact of HLA Matching and HLA Antibodies in Organ Transplantation: a Collaborative Transplant Study View

Caner Susal and Gerhard Opelz



16. Screening for Antibodies Against MICA by Luminex Flow Cytometry

Yizhou Zou and Peter Stastny



17. HLA Antibody Detection and Characterization by Solid Phase Immunoassays - Methods and Pitfalls

Andrea A. Zachary, Renato M. Vega, Donna P. Lucas, and Mary S. Leffell



18. Detection and Characterisation of Alloreactive T Cells

Mandvi Bharadwaj, Nicole A. Mifsud, and James McCluskey



19. Detection of Allo-HLA Crossreactivity by Viral Specific Memory T-Cell Clones using Single HLA Transfected K562 Cells

Lloyd J. D'Orsogna, Ellen M.W. van der Meer-Prins, Yvonne M. Zoet, Dave L. Roelen, Ilias I.N. Doxiadis, and Frans H.J. Claas



20. Separation and Cryopreservation of Lymphocytes from Spleen and Lymph Node

Gabriella Tassone and Samantha J. Fidler



21. Crossmatching by Complement-Dependent Lymphocytotoxicity (CDC)

Samantha J. Fidler



22. The Lymphocyte Crossmatch by Flow Cytometry for Kidney Transplantation

Jonathan Downing



23. Overview of the Killer Cell Immunoglobulin-Like Receptor (KIR) System

Raja Rajalingam



24. KIR Typing by Non Sequencing Methods: Polymerase-Chain Reaction with Sequence-Specific Primers (PCR-SSP)

David Ordonez, Manuela Moraru, Natalia Gomez-Lozano, Elisa Cisneros, and Carlos Vilches



25. Killer Cell Immunoglobulin-Like Receptors (KIR) Typing by DNA Sequencing

Lihua Hou, Minghua Chen, Noriko Steiner, Kanthi Kariyawasam, Jennifer Ng, and Carolyn K. Hurley



26. An Overview of Methods Required to Evaluate Donor NK Cell Alloreactivity for Haploidentical Haemopoietic Stem Cell Transplantation

Andrea Velardi



27. The Detection of NK Cell Alloreactivity by Flow Cytometric CD107a Assay

Dianne De Santis, Bree Foley, Campbell S. Wit, and Frank T. Christiansen



28. Clinical Production and Therapeutic Applications of Alloreactive Natural Killer Cells

David H. McKenna, Diane M. Kadidlo, Sarah Cooley, and Jeffrey S. Miller



29. Minor Histocompatibility Antigen Typing by DNA Sequencing for Clinical Practice in Hematopoietic Stem-Cell Transplantation

Eric Spierings and Els Goulmy



30. Donor Registries and Search Strategies

Carolyn K. Hurley, Machteld Oudshoorn, and Michelle Setterholm



31. Cytokine Gene Polymorphisms: Methods of Detection and Biological Significance

Gurvinder Kaur and Narinder Mehra



32. IMGT (R) Tools for the Nucleotide Analysis of Immunoglobulin (IG) and T Cell Receptor (TR) V-(D)-J Repertoires, Polymorphisms, and IG Mutations: IMGT/V-QUEST and IMGT/HighV-QUEST for NGS

Eltaf Alamyar, Patrice Duroux, Marie-Paule Lefranc, and Veronique Giudicelli



33. IMGT/DomainGapAlign: the IMGT (R) Tool for the Analysis of IG, TR, MH, IgSF and MhSF Domain Amino Acid Polymorphism

Francois Ehrenman and Marie-Paule Lefranc



34. Human Gm, Km and Am Allotypes and their Molecular Characterization: a Remarkable Demonstration of Polymorphism

Marie-Paule Lefranc and Gerard Lefranc
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Review Text

From the reviews:
"This book is a comprehensive volume covering a wide-range of topics related to HLA in the clinical setting. ... this book is an extremely useful reference for those working in the field of transplantation, or with an interest in the clinical applications of HLA. The introductory chapters to various topics are clear and provide a good amount of detail and are well referenced for those who wish to read further on the subject." (Vivien Hanson, Immunology News, February, 2013)
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Review quote

From the reviews:

"This book is a comprehensive volume covering a wide-range of topics related to HLA in the clinical setting. ... this book is an extremely useful reference for those working in the field of transplantation, or with an interest in the clinical applications of HLA. The introductory chapters to various topics are clear and provide a good amount of detail and are well referenced for those who wish to read further on the subject." (Vivien Hanson, Immunology News, February, 2013)
show more