Immunochemical Techniques : Laboratory Manual
Recently, there has been an explosion of immunochemical techniques and their application to biological sciences in research and industry. This manual, designed for courses and workshops in immunotechniques, contains student-tested protocols for affinity chromatography, Western blotting, ion-exchange chromatography, immunostaining of cells, ELISAs, and more than 30 other methods. It also provides extensive discussion of principles underlying these techniques and information about their wider applications. It includes features such as: conveniently combbound for the laboratory; large 9-1/4" x 7-1/2" format with marginal notes pinpointing critical steps, possible dangers, and workable alternatives. It contains sure-fire, student-tested exercises. It explains the theoretical basis for the experiments. It gives students experience with a wide variety of methods. It provides detailed information for instructors. It lists required materials at the beginning of each experiment, and offers helpful appendices with money-saving methods to produce materials for the course.
- Spiral bound | 188 pages
- 190.5 x 248.92 x 12.7mm | 385.55g
- 25 Jan 1993
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Table of contents
Methods Locator. Preface. Notes to Students and Instructors: Student Background for Laboratory, Immunochemistry Supplies, Antibody Requirements for Experiments, Suggested Schedule of Experiments. Background: Review of the Immune System and Immunoglobulin Structure. Experiments: Preparation of Serum from Blood. Purification and Analysis of IgG. Quantitative Precipitin Curve. Precipitation Analysis in Gel. Affinity Chromatography of Antibodies. Labeling Antibodies. Protein Blotting. Immunoprecipitation with Staphylococcal Protein A. Immunocytochemical Staining of Lymphocytes. Enzyme-Linked Immunosorbent Assay: ELISA. Appendices: Chemistries and Procedures for Anninity Chromatography. Buffers. PAGE Reagent and Staining Procedures. Western Blotting Reagents and Staining Procedures. Suppliers of Equipment and Chemical Reagents. Saturated Ammonium Sulfate Table. Radioiodination of Proteins. Immunization Procedure. Detergent Solubilization of Proteins. Index.